VIABILITY OF THE VASCULARLY PERFUSED, RECIRCULATING RAT INTESTINE AND INTESTINE-LIVER PREPARATIONS
VIABILITY OF THE VASCULARLY PERFUSED, RECIRCULATING RAT INTESTINE AND INTESTINE-LIVER PREPARATIONS
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DOI：
10.1152/ajpgi.1989.257.2.g249
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发表时间：
1989-08-01
影响因子：
--
通讯作者：
PANG, KS
中科院分区：
文献类型：
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作者：
HIRAYAMA, H;XU, X;PANG, KS
在禁食和非禁食大鼠中评价了血管灌注、再循环原位大鼠肠（I）和精氨酸-肝脏（IL）制备物的功能和稳定性，因为这些技术可容易地应用于药物代谢研究。通过上级肠系膜动脉用血液介质（7.5 ml/min）灌注大鼠肠，静脉流出物排入门静脉，其与肝动脉流量（2.5 ml/min）一起构成到肝脏的总血流量（10 ml/min）。观察到肠膜完整性的维持。将这些标记物推注到十二指肠幽门端后，[14 C]葡萄糖吸收迅速，浓度梯度和缺乏[3 H]-聚乙二醇4000（PEG 4000，< 4%），再循环I和IL制剂吸收伊文思蓝。显示肠和肝功能稳定的其他指标如下：储库灌注液量的保存，灌注压、胆汁流量和血红蛋白浓度的恒定性，肠葡萄糖利用和肝脏葡萄糖产生的证据，以及血清谷氨酸草酸转氨酶缺乏显著渗漏。肠和肝脏消耗氧气在相对恒定的速度，但消耗率为禁食组织（I或L）显着高于非禁食组织。这些结果表明，血管灌注的I和IL制剂维持在一个可行的和稳定的状态为2小时的灌注期。
Function and stability of vascularly perfused, recirculating in situ rat intestine (I) and intestine-liver (IL) preparations were evaluated in fasted and nonfasted rats because these techniques may be readily applied in drug metabolism studies. The rat intestine was perfused with blood medium (7.5 ml/min) via the superior mesenteric artery, with the venous outflow draining into the portal vein, which, together with hepatic arterial flow (2.5 ml/min), constituted the total blood flow (10 ml/min) to the liver. Maintenance of intestinal membrane integrity was observed. Rapid [14C]glucose absorption against a concentration gradient and a lack of [3H]-polyethylene glycol 4000 (PEG 4000, < 4%) and Evans blue absorption by the recirculating I and IL preparations resulted after bolus injections of these markers into the pyloric end of the duodenum. Other indexes that revealed stable intestinal and liver functions were the following: preservation of reservoir perfusate volume, constancy in perfusion pressure, bile flow, and hemoglobin concentrations, evidence of intestinal glucose utilization and liver glucose production, and a lack of significant leakage of serum glutamic oxalic transaminase. The intestine and liver consumed oxygen at relatively constant rates, but the consumption rates for the fasted tissues (I or L) were significantly higher than those for nonfasted tissues. These results indicate that the vascularly perfused I and IL preparations were maintained in a viable and stable state for a 2-h perfusion period.