Effects of epidermal growth factor and transforming growth factor-beta on insulin-induced differentiation in rat dental pulp cells.
Effects of epidermal growth factor and transforming growth factor-beta on insulin-induced differentiation in rat dental pulp cells.
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发表时间：
1992
影响因子：
3
通讯作者：
R. Liang;S. Nishimura;S. Sato
中科院分区：
文献类型：
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作者：
R. Liang;S. Nishimura;S. Sato
建立牙髓细胞系(RPC-C2a)，研究胰岛素对牙本质形成的调节作用。胰岛素促进细胞碱性磷酸酶活力和[2，3-~3H]-Pro掺入胶原酶可消化蛋白，而[~3H]-胸腺嘧啶核苷掺入则被胰岛素抑制。胰岛素对碱性磷酸酶活性的增强作用可被表皮生长因子(EGF)或转化生长因子-β(TGF-β)抑制。当胰岛素与EGF联合应用时，胰岛素对胶原合成的刺激作用也受到抑制，但加入转化生长因子-β后，其促进胶原合成的作用增强。胰岛素对[~3H]-胸腺嘧啶核苷掺入的抑制作用可被EGF增强，但单独使用EGF可明显增强这种抑制作用，而加入转化生长因子-β对胰岛素的作用无明显影响。这些结果提示胰岛素可能与牙本质形成过程中牙髓细胞的分化有关，EGF或转化生长因子-β对胰岛素的作用有调节作用。
An established pulp cell line (RPC-C2A) was used to study the regulatory effect of insulin on dentinogenesis. Insulin increased alkaline phosphatase activity and the incorporation of [2,3-3H]-proline into collagenase-digestible protein, whereas [3H]-thymidine incorporation by the cells was inhibited by insulin. The enhancing effect of insulin on alkaline phosphatase activity was inhibited by epidermal growth factor (EGF) or transforming growth factor-beta (TGF-beta). The stimulatory effect of insulin on collagen synthesis was also inhibited when insulin was combined with EGF, but was accelerated by the addition of TGF-beta. Inhibitory effects of insulin on the [3H]-thymidine incorporation were potentiated by EGF, though EGF alone strongly increased the effect; whereas the addition of TGF-beta had no significant effect on the insulin action. These findings suggest that insulin may be concerned with the differentiation of pulp cells in dentinogenesis and that EGF or TGF-beta regulate the insulin effects.