Phorbol ester stimulates progesterone production by isolated bovine luteal cells.
Phorbol ester stimulates progesterone production by isolated bovine luteal cells.
复制标题
佛波酯刺激分离的牛黄体细胞产生黄体酮。
作者：
B. Brunswig;A. Mukhopadhyay;L. Budnik;H. Bohnet;F. Leidenberger
肿瘤启动子phorbol 12-肉豆蔻酸酯-13-乙酸酯（PMA）被认为可以通过激活Ca++-和磷脂依赖的蛋白激酶（蛋白激酶C）来调节几种甾体生成组织的反应。这种激酶的存在已经在牛黄体中得到证实，尽管它在这些细胞的类固醇生成中的作用尚不清楚。我们在这里报告了PMA对体外酶分散牛黄体细胞产生黄体酮的影响。PMA （1-50 nM）使黄体细胞产生的黄体酮量呈剂量和时间相关增加。当PMA为10 nM时，达到了最大增产效果。较高浓度的PMA导致甾体生成下降到接近基础水平。而非促进衍生物4 α -苯酚12,13-二癸酸酯则没有作用。pma诱导的黄体酮产生的刺激与cAMP水平的变化无关。PMA与次优剂量的人CG、8Br-cAMP、霍乱毒素或福斯克林一起添加，显著增加了孕酮的产生量。PMA和人cg诱导的甾体生成对环己亚胺抑制敏感。外源性孕烯醇酮或25-羟胆固醇向黄体酮的转化不受PMA的影响。我们得出结论，纳米摩尔浓度的PMA能够刺激牛黄体细胞产生黄体酮，并且PMA的作用部位远于cAMP的形成，但在孕烯醇酮的形成之前。PMA在黄体细胞中的作用可能与其激活蛋白激酶C的能力有关，因为二酰基甘油可以模拟PMA的类固醇生成作用。
The tumor promoter, phorbol 12-myristate-13-acetate (PMA), is known to modulate the response of several steroidogenic tissues presumably by activating a Ca++- and phospholipid-dependent protein kinase (protein kinase C). The presence of this kinase has been demonstrated in bovine corpus luteum, although its role in steroidogenesis by these cells is unknown. We report here the effects of PMA on progesterone production by the enzymically dispersed bovine luteal cells in vitro. PMA (1-50 nM) produced a dose- and time-related increase in progesterone production by the luteal cells. The maximum stimulation was achieved with 10 nM PMA. Higher concentrations of PMA led to a decline of steroidogenesis close to the basal level. A nonpromoting derivative, 4 alpha-phorbol 12,13-didecanoate had no effect. The PMA-induced stimulation of progesterone production was not associated with a change in the cAMP level. PMA added together with suboptimal doses of human CG, 8Br-cAMP, cholera toxin, or forskolin significantly increased the amount of progesterone produced. PMA as well as human CG-induced steroidogenesis was sensitive to cycloheximide inhibition. The conversion of exogenous pregnenolone or 25-hydroxycholesterol to progesterone was not altered by PMA. We conclude that PMA at nanomolar concentrations is able to stimulate progesterone production by bovine luteal cells and that the site of action of PMA is distal to the formation of cAMP but before the formation of pregnenolone. The observed effects of PMA in luteal cells are probably linked to its ability to activate protein kinase C, since a diacylglycerol could mimic the steroidogenic action of PMA.
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影响因子：
--
作者：
Diamond, L;O'Brien, T G;Baird, W M
通讯作者：
Baird, W M
大鼠黄体线粒体和分散细胞对 25-羟基胆固醇的代谢。
影响因子：
4.8
作者：
Toaff,ME;Schleyer,H;Strauss3rd,JF
通讯作者：
Strauss3rd,JF
促进肿瘤的佛波酯影响大鼠垂体细胞催乳素和生长激素的产生。
影响因子：
4.8
作者：
Osborne,R;TashjianJr,AH
通讯作者：
TashjianJr,AH
DOI：
10.1073/pnas.80.1.36
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
NIEDEL, JE;KUHN, LJ;VANDENBARK, GR
通讯作者：
VANDENBARK, GR
佛波酯在体外抑制卵巢和睾丸类固醇生成。
影响因子：
11.2
作者：
WelshJr,TH;Jones,PB;Hsueh,AJ
通讯作者：
Hsueh,AJ