INFLUENCE OF AN INTERMITTENT COMPRESSIVE FORCE ON MATRIX PROTEIN EXPRESSION BY ROS 17/2.8 CELLS, WITH SELECTIVE STIMULATION OF OSTEOPONTIN
INFLUENCE OF AN INTERMITTENT COMPRESSIVE FORCE ON MATRIX PROTEIN EXPRESSION BY ROS 17/2.8 CELLS, WITH SELECTIVE STIMULATION OF OSTEOPONTIN
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DOI：
10.1016/0003-9969(93)90150-k
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发表时间：
1993-01-01
影响因子：
3
通讯作者：
SODEK, J
中科院分区：
文献类型：
--
作者：
KUBOTA, T;YAMAUCHI, M;SODEK, J
本研究的目的是确定骨细胞对物理压力的反应。间歇压缩力（ICF） 13 kPa，以18 cycles/min的速度施加于ROS 17/2.8细胞。48h后，用[S-35]-蛋氨酸或[(PO4)-P-32]标记细胞。与对照细胞相比，在此期间应用ICF并没有改变I型胶原蛋白、纤维连接蛋白或骨SPARC（骨连接蛋白）的合成。然而，碱性磷酸酶的活性增加了1.5倍，并且(PO4)- p -32标记的75-kDa磷酸化蛋白的合成增加了1.4倍，特异性抗体免疫沉淀识别为骨桥蛋白。此外，应用ICF后12小时内开始观察到骨桥蛋白mRNA的增加。与ICF相关的骨桥蛋白表达的选择性增加可能在生长发育期间骨组织的重塑和对功能力的反应中很重要。
The purpose of this study was to determine the response of bone cells to physical stress. Intermittent compressive force (ICF) was applied to 13 kPa to subconfluent ROS 17/2.8 cells at 18 cycles/min. After 48 h of this application, the cells were labelled with [S-35]-methionine or [(PO4)-P-32]. Application of ICF over this time did not alter the synthesis of type I collagen, fibronectin or bone SPARC (osteonectin) compared to that of control cells. However, the activity of alkaline phosphatase was increased 1.5-fold, and the synthesis of a (PO4)-P-32-labelled, 75-kDa phosphoprotein, recognized as osteopontin by immunoprecipitation with specific antibodies, was increased 1.4-fold. Also, an increase in osteopontin mRNA starting within 12 h of ICF application was observed. The selective increase in osteopontin expression associated with ICF may be important in the remodelling of bone tissues during growth and development and in response to functional forces.