EXPRESSION OF MONOCYTE CHEMOTACTIC AND ACTIVATING FACTOR IN RHEUMATOID-ARTHRITIS - REGULATION OF ITS PRODUCTION IN SYNOVIAL-CELLS BY INTERLEUKIN-1 AND TUMOR-NECROSIS-FACTOR
EXPRESSION OF MONOCYTE CHEMOTACTIC AND ACTIVATING FACTOR IN RHEUMATOID-ARTHRITIS - REGULATION OF ITS PRODUCTION IN SYNOVIAL-CELLS BY INTERLEUKIN-1 AND TUMOR-NECROSIS-FACTOR
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DOI：
10.1002/art.1780360605
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发表时间：
1993-06-01
影响因子：
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通讯作者：
MATSUSHIMA, K
中科院分区：
文献类型：
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作者：
AKAHOSHI, T;WADA, C;MATSUSHIMA, K
目标。目的探讨单核细胞趋化活化因子(MCAF)在类风湿关节炎(RA)患者关节内巨噬细胞聚集中的作用。采用放射免疫法测定MCAF。Northern印迹和逆转录聚合酶链式反应检测MCAF基因的表达。将重组人MCAF注射入兔关节内，观察MCAF对巨噬细胞浸润的影响。类风湿关节炎患者关节液中MCAF水平较高。新鲜分离的滑液细胞表达MCAF信使RNA。在体外，成纤维细胞样滑膜细胞表达MCAF mRNA，并在白细胞介素1(IL-1)和肿瘤坏死因子的作用下分泌MCAF。IL-1还可促进兔滑膜组织中MCAF基因的表达。MCAF可引起兔滑膜组织中巨噬细胞的明显浸润。我们的发现表明，MCAF可能有助于炎症的类风湿关节中巨噬细胞的聚集。
Objective. To investigate whether monocyte chemotactic and activating factor (MCAF) contributes to the accumulation of macrophages in the joints of patients with rheumatoid arthritis (RA).Methods. MCAF was measured by radioimmunoassay. MCAF gene expression was determined by Northern blotting and reverse-transcriptase polymerase chain reaction. Recombinant human MCAF was injected into rabbit joints to evaluate the effect of MCAF on infiltration of macrophages.Results. High levels of MCAF were detected in synovial fluid from patients with RA. Cells freshly isolated from synovial fluid expressed MCAF messenger RNA (mRNA). Fibroblast-like synoviocytes were found to express MCAF mRNA and to secrete MCAF in response to interleukin-1 (IL-1) and tumor necrosis factor in vitro. IL-1 also promoted MCAF gene expression in rabbit synovial tissue in vivo. MCAF caused marked infiltration of macrophages in rabbit synovial tissue.Conclusion. Our findings suggest that MCAF may contribute to the accumulation of macrophages in inflamed rheumatoid joints.