Changes in mRNA and protein levels of human HtrA1, HtrA2 and HtrA3 in ovarian cancer
Changes in mRNA and protein levels of human HtrA1, HtrA2 and HtrA3 in ovarian cancer
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DOI：
10.1016/j.clinbiochem.2008.01.004
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发表时间：
2008-05-01
影响因子：
2.8
通讯作者：
Lipinska, Barbara
中科院分区：
文献类型：
--
作者：
Narklewicz, Joanna;Klasa-Mazurkiewicz, Dagmara;Lipinska, Barbara
目的：方法：采用半定量RT-PCR和Western-blotting方法检测19例正常卵巢、20例良性卵巢肿瘤、7例交界性卵巢肿瘤、44例卵巢癌和8例Krukenberg瘤组织中HtrA 1、HtrA 2、HtrA 3和TGF-β 1基因的表达。与正常组织相比，观察到卵巢肿瘤中HtrA 1和HtrA 3表达的统计学显著降低。结果表明，所有肿瘤组织中HtrA 3 mRNA和蛋白水平均显著降低，30%的恶性肿瘤组织中HtrA 3蛋白缺失，与良性肿瘤相比，恶性肿瘤组织中HtrA 1 mRNA、HtrA 3 mRNA和蛋白水平均显著降低。HtrA 2在肿瘤组织中的表达略有下降。TGF-β 1在肿瘤组织中的表达与对照组织相比无显著差异。结论：我们的结果显示HtrA I和HtrA 3基因在不同类型的卵巢肿瘤中表达下调，并进一步证明这些基因可能具有肿瘤抑制作用。(c)2008年加拿大临床化学家协会。爱思唯尔公司出版All rights reserved.
Objectives: Expression of human HtrA1, HtrA2, HtrA3 and TGF-beta 1 genes was examined in ovarian tissue specimens including 19 normal ovaries, 20 benign tumors, 7 borderline tumors, 44 cancers and 8 Krukenberg tumors.Design and methods: mRNA and protein levels were evaluated by semi-quantitative RT-PCR and Western-blotting methods, respectively.Results: A statistically significant decrease of HtrA1 and HtrA3 expression in ovarian tumors comparing to normal tissues was observed. A dramatic decrease of HtrA3 mRNA and protein levels in all tumor tissue groups, and a loss of HtrA3 protein in 30% malignant tumors were found. A significant decrease of HtrA1 mRNA, and of HtrA3 mRNA and protein in malignant tumors compared to benign tumors was revealed. HtrA2 expression in tumor tissues was slightly decreased. Expression of TGF-beta 1 in tumor tissues was not significantly different compared to control tissues.Conclusions: Our results show downregulation of HtrA I and HtrA3 genes' expression in different types of ovarian tumors and give additional evidence that these genes may function as tumor suppressors. (c) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.