Synaptic Organization in the Adult Drosophila Mushroom Body Calyx
Synaptic Organization in the Adult Drosophila Mushroom Body Calyx
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DOI：
10.1002/cne.22184
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发表时间：
2009-12-20
影响因子：
2.5
通讯作者：
Tavosanis, Gaia
中科院分区：
文献类型：
--
作者：
Leiss, Florian;Groh, Claudia;Tavosanis, Gaia
昆虫蘑菇体是嗅觉联想学习的关键。我们对成年黑腹果蝇（Drosophila melanogaster）花萼的突触组织进行了广泛的定量描述，花萼是蘑菇体的主要嗅觉输入区域。通过使用高分辨率共聚焦显微镜，基于电子显微镜的三维重建，以及贡献于花萼的神经元群体的遗传标记，我们解决了天线叶投射神经元的大胆碱能束与蘑菇体固有神经元凯尼恩细胞树突之间的精确联系。在整个花萼中，这些成分构成称为微肾小球的突触复合体。通过单细胞标记，我们发现每个凯尼恩细胞的爪状树突状特化高度富集丝状肌动蛋白，这表明这可能是塑料重组的一个位点。事实上，Lim激酶（LimK）在Kenyon细胞中的过度表达改变了微肾小球的形状。共聚焦显微镜和电子显微镜显示，每个凯尼恩细胞爪包住一个投射神经元的单个钮扣。每个钮扣都由许多类似爪状的特化物以及γ -氨基丁酸阳性神经元的轮廓连接。参与不同类型记忆的Kenyon细胞的不同群体的树突在花萼内部分分离，并有助于形成不同的微肾小球亚群。然而，我们的分析表明，投射神经元钮扣可以接触到不止一种类型的凯尼恩细胞。这些发现为嗅觉通路的功能分析提供了重要的基础，包括联想嗅觉记忆的形成。[j] .中国生物医学工程学报，2009。(C) 2009 Wiley-Liss, Inc。
Insect mushroom bodies are critical for olfactory associative learning. We have carried out an extensive quantitative description of the synaptic organization of the calyx of adult Drosophila melanogaster, the main olfactory input region of the mushroom body. By using high-resolution confocal microscopy, electron microscopy-based three-dimensional reconstructions, and genetic labeling of the neuronal populations contributing to the calyx, we resolved the precise connections between large cholinergic boutons of antennal lobe projection neurons and the dendrites of Kenyon cells, the mushroom body intrinsic neurons. Throughout the calyx, these elements constitute synaptic complexes called microglomeruli. By single-cell labeling, we show that each Kenyon cell's claw-like dendritic specialization is highly enriched in filamentous actin, suggesting that this might be a site of plastic reorganization. In fact, Lim kinase (LimK) overexpression in the Kenyon cells modifies the shape of the microglomeruli. Confocal and electron microscopy indicate that each Kenyon cell claw enwraps a single bouton of a projection neuron. Each bouton is contacted by a number of such claw-like specializations as well as profiles of gamma-aminobutyric acid-positive neurons. The dendrites of distinct populations of Kenyon cells involved in different types of memory are partially segregated within the calyx and contribute to different subsets of microglomeruli. Our analysis suggests, though, that projection neuron boutons can contact more than one type of Kenyon cell. These findings represent an important basis for the functional analysis of the olfactory pathway, including the formation of associative olfactory memories. J. Comp. Neurol. 517:808-824 2009. (C) 2009 Wiley-Liss, Inc.