Five newly established oesophageal carcinoma cell lines: phenotypic and immunological characterization.
Five newly established oesophageal carcinoma cell lines: phenotypic and immunological characterization.
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DOI：
10.1038/bjc.1997.42
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发表时间：
1997
影响因子：
8.8
通讯作者：
Matthews HR
中科院分区：
文献类型：
--
作者：
Rockett JC;Larkin K;Darnton SJ;Morris AG;Matthews HR
已尝试从40例切除的食道癌中分离出永久性细胞系。从三例腺癌、一例混合癌和一例鳞癌中建立了五个长期系。对从它们衍生的品系和克隆进行了分子和细胞分析。核型分析表明，无性系之间存在遗传变异。人类白细胞抗原-A、-B和-C呈结构性表达，但不表达HLA-DR。ICAM-1表达的表型可能是在适应长期培养的过程中出现的。所有品系都能对干扰素-伽马(干扰素-伽马)产生反应，并都能产生转化生长因子β1(转化生长因子β1)。有两个品系对后者的生长抑制作用具有抵抗性，这可能是导致恶性的原因之一。预计这些起源于不同组织学肿瘤的株系将为这些侵袭性肿瘤的研究和治疗提供有价值的、持续的资源。
The derivation of permanent cell lines from 40 resected oesophageal carcinomas has been attempted. Five long-term lines have been established from three adenocarcinomas, one mixed carcinoma and one squamous carcinoma. Molecular and cellular analyses have been carried out on the lines and clones derived from them. Karyotype analysis indicates genetic variation among the clones. HLA-A, -B and -C is expressed constitutively, but not HLA-DR. ICAM-1-expressing phenotypes may have arisen during adaptation to long-term culture. All lines are capable of response to interferon-gamma (IFN-gamma) and all produce transforming growth factor beta 1 (TGF-beta 1). Two lines are resistant to the inhibitory growth effects of the latter, possibly contributing to malignancy. It is anticipated that these lines, originating from histologically different carcinomas, will provide a valuable, continuous resource for the investigation and treatment of these aggressive tumours.