Rapid detection of food-borne pathogens by using molecular techniques
Rapid detection of food-borne pathogens by using molecular techniques
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DOI：
10.1099/jmm.0.45687-0
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发表时间：
2005-01-01
影响因子：
3
通讯作者：
Jamil, K
中科院分区：
文献类型：
--
作者：
Naravaneni, R;Jamil, K
传统的食源性致病菌鉴定方法费时费力，因此有必要开发快速鉴定食源性致病菌的创新方法。分子克隆和重组DNA技术的最新进展使食品中病原体的检测发生了革命性的变化。本研究建立了一种基于聚合酶链式反应的快速鉴定食源性致病菌沙门氏菌和大肠杆菌的技术。在此基础上设计了合适的引物。沙门氏菌特异基因fimA和致病性大肠埃希氏菌基因afa用于扩增。用琼脂糖凝胶电泳法和溴化乙锭染色鉴定扩增产物。与标记DNA比较，扩增产物大小为120bp。这些研究已经证实，FIMA和ALA引物分别对环境样品中的沙门氏菌和致病性大肠杆菌具有特异性。从而建立了一种快速、灵敏、可靠的检测沙门氏菌和致病性大肠杆菌的方法。
Traditional methods of identification of food-borne pathogens, which cause disease in humans, are time-consuming and laborious, so there is a need for the development of innovative methods for the rapid identification of food-borne pathogens. Recent advances in molecular cloning and recombinant DNA techniques have revolutionized the detection at pathogens in foods. In this study the development of a PCR-based technique for the rapid identification of the food-borne pathogens Salmonella and Escherichia coli was undertaken. Suitable primers were designed based or. specific gene fimA of Salmonella and gene afa of pathogenic E coli for amplification. Agarose gel electrophoresis and subsequent staining with ethidium bromide were used for the identification of PCR products. The size of the amplified product was 120 bp as shown by comparison with marker DNA. These studies have established that fimA and ala primers were specific for detecting Salmonella and pathogenic E coli, respectively, in the environmental samples. Thus a rapid, sensitive and reliable technique for the detection of Salmonella and pathogenic E coli was developed.