Effects of single-walled carbon nanotubes on the functions of plasma proteins and potentials in vascular prostheses
Effects of single-walled carbon nanotubes on the functions of plasma proteins and potentials in vascular prostheses
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DOI：
10.1016/j.nano.2005.03.003
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发表时间：
2005-06-01
影响因子：
5.4
通讯作者：
Xie, Sishen
中科院分区：
文献类型：
--
作者：
Meng, Jie;Song, Li;Xie, Sishen
背景资料：非织造单壁纳米管（SWNTs）是一种具有纳米形貌宏观表面和纯碳成分的新型材料，可作为血液接触环境中植入物的涂层。方法：采用扫描电子显微镜（SEM）、能量色散X射线和直接酶联免疫吸附试验研究了白蛋白、纤维蛋白原和新鲜血浆在SWNTs膜上的吸附行为。血小板粘附和活化进行了研究，在体外使用SEM观察，并通过流式细胞仪分析PAC-1，它结合到GPIIb/IIIa上表达的活化platelet.Results：非织造单壁碳纳米管清楚地显示出更大的吸附偏好纤维蛋白原比白蛋白。然而，通过SEM未观察到粘附的血小板。流式细胞仪分析结果进一步证明了无纺单壁碳纳米管引起的血小板GPIIb/ IIIa表达的极低水平。结论：吸附纤维蛋白原介导血小板识别、粘附、活化和聚集的功能被显著抑制，导致血小板粘附和活化水平极低。(C)2005年爱思唯尔公司All rights reserved.
Background: Nonwoven single-walled nanotubes (SWNTs) are novel materials with a nanotopography macroscopic surface and pure-carbon composition, which may serve as coatings on implants in blood-contact environments.Methods: The adsorption behavior of albumin, fibrinogen, and fresh plasma on SWNT membranes was studied using scanning electron microscopy (SEM), energy-dispersive x-ray, and direct enzymelinked immunosorbent assay, respectively. Platelet adhesion and activation were investigated in vitro using SEM observation, and via flow cytometry analysis by PAC-1, which binds to GPIIb/IIIa expressed on activated platelets.Results: Nonwoven SWNTs clearly displayed greater adsorption preference for fibrinogen than for albumin. However, adhered platelets were not observed by SEM. Results obtained from flow cytometry analysis further proved very low expression of GPIIb/ IIIa on platelets caused by nonwoven SWNTs.Conclusion: The function of adsorbed fibrinogen to mediate platelet recognition, adhesion, activation, and aggregation was significantly suppressed, which induced extremely low levels of platelet adhesion and activation. (C) 2005 Elsevier Inc. All rights reserved.