Time course of the diameter of the major cerebral arteries after subarachnoid hemorrhage using corrosion cast technique
Time course of the diameter of the major cerebral arteries after subarachnoid hemorrhage using corrosion cast technique
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DOI：
10.1179/016164103101201535
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发表时间：
2003-06
影响因子：
1.9
通讯作者：
S. Ono;I. Date;K. Onoda;T. Ohmoto
中科院分区：
文献类型：
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作者：
S. Ono;I. Date;K. Onoda;T. Ohmoto
摘要 在本报告中，我们研究了腐蚀铸型法是否也适用于产生蛛网膜下腔出血（SAH）的大鼠主动脉的测量和估计。此外，我们还检查了蛛网膜下腔出血后大鼠主动脉的直径。雄性Sprague-Dawley大鼠小脑延髓池注射自体血0.3ml，建立SAH模型。 SAH后10分钟、30分钟、1小时、4小时、8小时、1天、2天、3天、5天和7天进行半聚合铸塑介质的灌注。大脑在 10% NaOH 溶液中腐蚀。然后使用扫描电子显微镜 (SEM) 测量 BA 和其他主要动脉。还进行了肉眼观察和苏木精-伊红（HE）染色。使用腐蚀铸造方法，在 BA 中观察到双相收缩反应； SAH 后 30 分钟和 1 天分别观察到 8.3% 和 11.6% 的收缩。此外，慢性期几乎没有平滑肌或外膜增厚。相反，SAH后10分钟在颈内动脉和大脑中动脉中观察到扩张反应。宏观观察和HE染色显示，广泛的基底蛛网膜下腔血肿在第2天几乎消失。这些结果表明，在该模型中，SAH后一天发生的最小痉挛可以通过大鼠蛛网膜下腔容量较小和大脑基底主要血管周围的脑脊液快速冲洗来解释。此外，目前的数据还显示SAH后早期ICA和MCA的代偿性扩张。
Abstract In this report, we examined whether corrosion cast method is also applicable for the measurement and estimation of the rat major arteries in which subarachnoid hemorrhage (SAH) is produced. Additionally, we have examined the diameters of the rat major arteries following SAH. A total of 0.3 ml autologous blood was injected into the cisterna magna of male Sprague-Dawley rats for the SAH model. A perfusion of a semi-polymerized casting medium was performed, 10 min, 30 min, 1 h, 4 h, 8 h, 1 day, 2 days, 3 days, 5 days, and 7 days after SAH. The brains were corroded in a 10% NaOH solution. The BA and the other major arteries were then measured using scanning electron microscopy (SEM). Macroscopic observation and hematoxylin-eosin (HE) staining were also performed. Using the corrosion cast method, the biphasic contractile response was observed in the BA; 8.3% and 11.6% contractions were observed 30 min and 1 day after SAH, respectively. In addition, there was almost no smooth muscle or adventitial thickening in the chronic stage. In contrast, the dilative response was observed in the internal carotid artery and middle cerebral artery 10 min after SAH. Macroscopic findings and HE staining revealed that the extensive basal subarachnoid hematoma had almost disappeared by day 2. These results indicate that in this model, the minimal spasm, which occurs one day after SAH, can be explained by the small capacity of the rat subarachnoid space and the rapid cerebrospinal fluid washout around major vessels at the cerebral base. Moreover, the present data also show the compensatory dilatation in the ICA and MCA in the early stage after SAH.