The self-crosslinked ufasome of conjugated linoleic acid: Investigation of morphology, bilayer membrane and stability
The self-crosslinked ufasome of conjugated linoleic acid: Investigation of morphology, bilayer membrane and stability
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DOI：
10.1016/j.colsurfb.2014.08.028
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发表时间：
2014-11-01
影响因子：
5.8
通讯作者：
Ma, Lin
中科院分区：
文献类型：
--
作者：
Fan, Ye;Fang, Yun;Ma, Lin
不饱和脂肪酸脂质体（Ufasomes）由于其容易获得的不饱和脂肪酸和简单的组装策略而引起人们的兴趣。然而，脂质体的胶体不稳定性阻碍了其在药物载体和食品添加剂领域的应用。本论文以具有生物活性、组装和交联三重活性的共轭亚油酸（CIA）为分子构建单元，构建了超分子体，并对其进行了交联。首先，利用pH变化自组装CIA超微粒体，通过表面张力测定和酸碱滴定确定CIA超微粒体的适宜浓度和pH范围。随后，通过CIA分子中的共轭双键的自交联的CLA脂质体的脂质体内交联制备。使用透射电子显微镜（TEM）对自交联CIA超微粒体的形态进行成像，从中检测到20-50 nm的尺寸和2.7 +/-0.5 nm的双层厚度。最重要的是，基于不同脂肪酸的双层厚度的比较，自交联CIA超结构体的双层膜中的分子排列被命名为“并排”模型，而不是普通的“尾对尾”模型。借助于动态光散射测试，检查了自交联CIA脂质体的pH稳定性。最后，5-氟尿嘧啶从自交联CIA脂质体的体外释放结果表明，该过程是缓慢和可持续的。(C)2014爱思唯尔有限公司版权所有。
Unsaturated fatty acid liposomes (Ufasomes) have attracted interests because of the ready availability of unsaturated fatty acids and the simple assembly strategy. However, the colloidal instability of the ufasomes hinders them from applying in the fields of drug delivery and food additives. In the present work, conjugated linoleic acid (CIA) with triple activities of bioactive, assembling and crosslinking was employed as a new molecular building block to construct ufasome and afterwards crosslinked ufasome. First, CIA ufasome was self-assembled from CIA molecules in response to pH variation, and the suitable CIA concentrations and pH ranges were determined by surface tension measurement and acid-base titration. Subsequently, the self-crosslinked CLA ufasome was prepared by intra-ufasomal crosslinking of conjugated double bonds in the CIA molecules. The morphologies of the self-crosslinked CIA ufasomes were imaged using transmission electron microscopy (TEM), from which the size of 20-50 nm and the bilayer thickness of 2.7 +/- 0.5 nm were detected. Most importantly, based on the comparison of the bilayer thicknesses of the different fatty acids, the molecular arrangement in the bilayer membrane of the self-crosslinked CIA ufasome is named "side-by-side" model contrary to the ordinary "tail-to-tail" model. The pH stability of the self-crosslinked CIA ufasome was examined in virtue of dynamic light scattering tests. Finally, in vitro release results of 5-fluorouracil from the self-crosslinked CIA ufasome showed that the process was slow and sustainable. (C) 2014 Elsevier B.V. All rights reserved.