Transcriptome Analysis of Japanese Pear (Pyrus pyrifolia Nakai) Flower Buds Transitioning Through Endodormancy
Transcriptome Analysis of Japanese Pear (Pyrus pyrifolia Nakai) Flower Buds Transitioning Through Endodormancy
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DOI：
10.1093/pcp/pct067
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发表时间：
2013-07-01
影响因子：
4.9
通讯作者：
Moriguchi, Takaya
中科院分区：
文献类型：
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作者：
Bai, Songling;Saito, Takanori;Moriguchi, Takaya
利用RNA-SEQ技术分析了梨花蕾的转录本，并对其进行了比较。通过对不同数据库的BLAST搜索，在114,191个UNIGNES的从头组装中，76,995个UNIGNES被成功地注释。基因本体论(GO)分析表明，氧化还原酶在分子功能类别中得到了丰富，这一结果与先前观察到的过氧化氢浓度在气味释放过程中发生显著变化的结果一致。在与生物过程相关的GO类别中，DNA甲基化相关基因转录本的丰度在气味释放过程中也发生了显著变化，表明表观遗传调控参与了这一过程。京都基因和基因组百科全书(KEGG)途径分析也显示了参与各种植物激素代谢的基因转录丰度的变化。ABA和赤霉素生物合成基因均下调，而编码其降解酶的基因在内臭释放过程中上调。在乙烯途径中，编码乙烯生物合成限速酶的1-氨基环丙烷-1-羧酸合成酶(1-氨基环丙烷-1-羧酸合成酶)被诱导向内气味释放。所有这些结果表明，植物激素参与了内气味的释放。此外，随着内气味的释放，休眠相关MADS-box(DAM)基因的表达下调，尽管这些基因转录本的丰度变化并不像转录组分析所确定的那样显著。因此，对日本梨转录组从内源向生态香气转变过程的描述将为数据挖掘研究人员提供有用的信息，并将促进进一步的内源香气实验，特别是在玫瑰科果树上。
The transcriptomes of endodormant and ecodormant Japanese pear (Pyrus pyrifolia Nakai 'Kosui') flower buds were analyzed using RNA-seq technology and compared. Among de novo assembly of 114,191 unigenes, 76,995 unigenes were successfully annotated by BLAST searches against various databases. Gene Ontology (GO) enrichment analysis revealed that oxidoreductases were enriched in the molecular function category, a result consistent with previous observations of notable changes in hydrogen peroxide concentration during endodormancy release. In the GO categories related to biological process, the abundance of DNA methylation-related gene transcripts also significantly changed during endodormancy release, indicating the involvement of epigenetic regulation. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis also showed the changes in transcript abundance of genes involved in the metabolism of various phytohormones. Genes for both ABA and gibberellin biosynthesis were down-regulated, whereas the genes encoding their degradation enzymes were up-regulated during endodormancy release. In the ethylene pathway, 1-aminocyclopropane-1-carboxylate synthase (ACS), a gene encoding the rate-limiting enzyme for ethylene biosynthesis, was induced towards endodormancy release. All of these results indicated the involvement of phytohormones in endodormancy release. Furthermore, the expression of dormancy-associated MADS-box (DAM) genes was down-regulated concomitant with endodormancy release, although changes in the abundance of these gene transcripts were not as significant as those identified by transcriptome analysis. Consequently, characterization of the Japanese pear transcriptome during the transition from endormancy to ecodormancy will provide researchers with useful information for data mining and will facilitate further experiments on endodormancy especially in rosaceae fruit trees.