Three-dimensional fluorescence lifetime tomography
Three-dimensional fluorescence lifetime tomography
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DOI：
10.1118/1.1861160
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发表时间：
2005-04-01
期刊：
影响因子：
3.8
通讯作者：
Eppstein, MJ
中科院分区：
文献类型：
--
作者：
Godavarty, A;Sevick-Muraca, EM;Eppstein, MJ
使用分子靶向的寿命敏感的荧光造影剂的近红外荧光断层扫描在早期癌症诊断中具有应用。然而，虽然荧光寿命成像显微镜（FLIM）的测量被广泛用于显微镜和光谱学应用，荧光寿命断层扫描的医学成像的演示仅限于二维研究。在此，使用（i）增益调制的增强型电荷耦合器件（CCD）和调制的激光二极管成像系统，（ii）两种荧光造影剂，例如，吲哚菁绿色和3-3 '-二乙基硫代三碳菁碘化物的荧光寿命相差0.62 ns，和（iii）两阶段近似扩展卡尔曼滤波重建算法。在1.4-2 cm的目标深度处，在不同的目标与背景荧光吸收（70：1，100：1）和荧光寿命（1：1，2.1：1）对比下，在体模表面上获得相位和振幅的荧光测量。贝叶斯层析成像算法，以获得三维图像的寿命和吸收，由于荧光团。(c)2005年美国医学物理学家协会。
Near-infrared fluorescence tomography using molecularly targeted lifetime-sensitive, fluorescent contrast agents have applications for early-stage cancer diagnostics. Yet, although the measurement of fluorescent lifetime imaging microscopy (FLIM) is extensively used in microscopy and spectroscopy applications, demonstration of fluorescence lifetime tomography for medical imaging is limited to two-dimensional studies. Herein, the feasibility of three-dimensional fluorescence-lifetime tomography on clinically relevant phantom volumes is established, using (i) a gain-modulated intensified charge coupled device (CCD) and modulated laser diode imaging system, (ii) two fluorescent contrast agents, e.g., Indocyanine green and 3-3 '-Diethylthiatricarbocyanine iodide differing in their fluorescence lifetime by 0.62 ns, and (iii) a two stage approximate extended Kalman filter reconstruction algorithm. Fluorescence measurements of phase and amplitude were acquired on the phantom surface under different target to background fluorescence absorption (70: 1, 100:1) and fluorescence lifetime (1:1, 2.1:1) contrasts at target depths of 1.4-2 cm. The Bayesian tomography algorithm was employed to obtain three-dimensional images of lifetime and absorption owing to the fluorophores. (c) 2005 American Association of Physicists in Medicine.