COLLAGEN FIBRILLAR NETWORKS AS SKELETAL FRAMEWORKS - A DEMONSTRATION BY CELL-MACERATION SCANNING ELECTRON-MICROSCOPE METHOD
COLLAGEN FIBRILLAR NETWORKS AS SKELETAL FRAMEWORKS - A DEMONSTRATION BY CELL-MACERATION SCANNING ELECTRON-MICROSCOPE METHOD
复制标题
DOI：
10.1679/aohc.51.249
复制
发表时间：
1988-07-01
影响因子：
--
通讯作者：
KIKUTA, A
中科院分区：
文献类型：
--
作者：
OHTANI, O;USHIKI, T;KIKUTA, A
采用细胞浸渍/扫描电子显微镜（SEM）方法来显示各种组织的胶原纤维网络的排列。将固定的组织浸入NaOH（25 ℃）中，C）3-7天，随后在蒸馏水中冲洗，成功地去除了细胞成分，暴露了胶原原纤维，通过透射电子显微镜在其天然位置鉴定出胶原原纤维。制备物的SEM观察能够比其他方法（包括银浸渍法）更精确地证明胶原原纤维的三维结构。胶原原纤维，形成容纳单个心肌细胞的鞘，融合在一起，从而确保相邻心肌细胞的相等伸展并防止相邻细胞的滑动。单个骨骼肌纤维和神经纤维被以两个相反螺旋运行的胶原纤维的网状结构包裹。这种结构似乎在抵抗拉伸动力方面起着重要作用。在舌和指尖皮肤的上皮结缔组织连接处，交织的胶原纤维形成许多微脊，这可能为上皮提供了广泛的锚定。在肠粘膜中，胶原纤维网络直接低于绒毛上皮的基底层具有不均匀的孔。由于胶原纤维网络显示出特定于单个器官和组织的形态学特征，因此建议这种形成不仅构成骨骼框架，而且还为容纳在那里的那些细胞提供适合其活动的微环境。
A cell-maceration/scanning electron microscope (SEM) method was employed to demonstrate the arrangement of the collagen fibrillar network of various tissues. Immersion of fixed tissues in NaOH (25.degree. C) for 3-7 days, followed by rinsing in distilled water successfully removed the cellular elements, exposing collagen fibrils which were identified as such by transmission electron microscopy in their natural locations. SEM observations of the preparations are able to demonstrate the three-dimensional architecture of collagen fibrils much more precisely than other methods, including the silver impregnation method. Collagen fibrils, forming sheaths for housing individual cardiac myocytes, fused together, thus ensuring an equal stretch of contiguous myocytes and preventing the slippage of adjacent cells. Individual skeletal muscle fibers and nerve fibers were ensheathed by the meshwork of collagen fibrils running in two opposite helices. Such structues seem to play an important role in resisting the stretching impetus. At the epithelial-connective tissue junction of the tongue and fingertip skin, interwoven collagen fibrils formed numerous microridges which probably provide a broad anchorage for the epithelium. In the intestinal mucosa, the collagen fibrillar network immediately below the basal laminae of the villous epithelium possessed heterogeneous pores. As the collagen fibrillar network shows morphological features specific to individual organs and tissues, it is suggested that such formations not only constitute the skeletal framework but also provide those cells which are housed there with a microenvironment suitable for their activities.