Chlorophyll f synthesis by a super-rogue photosystem II complex.
Chlorophyll f synthesis by a super-rogue photosystem II complex.
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由超级流氓光系统 II 复合体合成叶绿素 f。
DOI：
10.1038/s41477-020-0616-4
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发表时间：
2020
期刊：
影响因子：
18
通讯作者：
Trinugroho JP
中科院分区：
文献类型：
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作者：
Trinugroho JP
某些蓝藻合成叶绿素分子（chld和Chlf），吸收太阳光谱的远红色区域，从而扩大光合有效辐射的光谱范围。这些远红叶绿素的合成和引入到植物的光合机构中可能会提高含氧光合作用的效率，特别是在富含远红的环境中，如冠层的下层。chl的产生需要chf亚基，也被称为PsbA4（参考文献）或超级流氓D1（参考文献），它是光系统II （PSII）的D1亚基的一种类似物，它与D2一起结合参与光驱动水氧化的辅因子。目前的观点认为，ChlF氧化Chlato Chlfin是一个同二聚体的ChlF反应中心（RC）复合物，是PSII异二聚体D1/D2 RC进化过程中缺失的一个环节（参考文献，）。然而，这一建议缺乏明确的生化支持。在这里，我们发现ChlF可以取代D1形成能够产生ChlF的修饰PSII配合物。值得注意的是，D1上仅两个残基的突变就能将进化氧气的PSII转化为氯合酶。总的来说，我们已经确定了一类新的PSII复合物，我们称之为“超级流氓”PSII，在色素生物合成而不是水氧化中具有意想不到的作用。
Certain cyanobacteria synthesize chlorophyll molecules (Chldand Chlf) that absorb in the far-red region of the solar spectrum, thereby extending the spectral range of photosynthetically active radiation,. The synthesis and introduction of these far-red chlorophylls into the photosynthetic apparatus of plants might improve the efficiency of oxygenic photosynthesis, especially in far-red enriched environments, such as in the lower regions of the canopy. Production of Chlfrequires the ChlF subunit, also known as PsbA4 (ref. ) or super-rogue D1 (ref. ), a paralogue of the D1 subunit of photosystem II (PSII) which, together with D2, bind cofactors involved in the light-driven oxidation of water. Current ideas suggest that ChlF oxidizes Chlato Chlfin a homodimeric ChlF reaction centre (RC) complex and represents a missing link in the evolution of the heterodimeric D1/D2 RC of PSII (refs.,). However, unambiguous biochemical support for this proposal is lacking. Here, we show that ChlF can substitute for D1 to form modified PSII complexes capable of producing Chlf. Remarkably, mutation of just two residues in D1 converts oxygen-evolving PSII into a Chlfsynthase. Overall, we have identified a new class of PSII complex, which we term ‘super-rogue’ PSII, with an unexpected role in pigment biosynthesis rather than water oxidation.