Maternal inheritance of transcripts from three Drosophila src-related genes.
Maternal inheritance of transcripts from three Drosophila src-related genes.
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三个果蝇 src 相关基因转录本的母系遗传。
DOI：
10.1093/nar/13.6.2153
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发表时间：
1985
影响因子：
14.9
通讯作者：
Bilodeau-Wentworth,D
中科院分区：
文献类型：
--
作者：
Wadsworth,SC;Madhavan,K;Bilodeau-Wentworth,D
果蝇基因组包含与v-src基因相关的三个主要序列。先前发表的分子研究已经证实了v-src和两个果蝇酶序列之间的结构同源性。我们对第三个v-src果蝇基因的一部分进行了测序，发现它与脊椎动物和果蝇src家族基因也有结构同源性。这些rc基因的RNA序列在前胚盘胚胎中存在，表明它们是母体来源的。随着胚胎发生的进行，每个rcRNA序列的水平下降。前胚盘基因转录物含有poly（A），并存在于多聚核糖体上，表明它们是功能性mRNA。由于果蝇src基因转录本是母系遗传的，我们还调查了它们在成年雌性中的分布。成年雌性的大部分转录本位于卵巢中。在雄性中仅检测到低水平的转录本。这些结果有力地表明，在早期胚胎发生过程中，可能在胚盘细胞核细胞化的时候，需要大量的src蛋白。
TheDrosophilagenome contains three major sequences related to the v-srcgene. Previously published molecular studies have confirmed the structural homology between v-srcand two of theDrosophilasequences. We have sequenced a portion of the third v-srcDrosophilagene and found that it also shares structural homology with vertebrate andDrosophila src-family genes. RNA sequences from each of thesrcgenes are present in pre-blastoderm embryos indicating that they are of maternal origin. As embryogenesis proceeds, the levels of each of thesrcRNA sequences decline. The pre-blastodermsrcgene transcripts contain poly(A) and are present on polyribosomes suggesting that they are functional mRNAs. Since theDrosophila srctranscripts were maternally inherited, we also investigeted their distribution in adult females. The majority of thesrctranscripts in adult females were contained in ovaries. Only low levels of the transcripts were detected in males. These results strongly suggest that an abundant supply ofsrcprotein is required during early embryogenesis, perhaps at the time of cellularization of the blastoderm nuclei.