Plant lamin-like proteins mediate chromatin tethering at the nuclear periphery
Plant lamin-like proteins mediate chromatin tethering at the nuclear periphery
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DOI：
10.1186/s13059-019-1694-3
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发表时间：
2019-04-30
期刊：
影响因子：
12.3
通讯作者：
Liu, Chang
中科院分区：
文献类型：
--
作者：
Hu, Bo;Wang, Nan;Liu, Chang
背景资料：核膜不仅是细胞核与细胞质之间的物理屏障，而且在调节基因组DNA的三维结构中起着关键作用。对于植物和动物，核周边是富含异染色质的功能区室。到目前为止，植物如何管理选择性地系留染色质在核peripher.Results：通过进行双色荧光原位杂交实验2C核，我们表明，在拟南芥中，特定的染色质定位在核周边需要植物核纤层蛋白样蛋白CROWDED NUCLEI 1（CRWN 1），CRWN 4，和DNA甲基化在CHG和CHH上下文。与染色体绘画和Hi-C分析，我们显示了全球衰减的空间染色质区室化和染色质定位模式在核周边的crwn 1和crwn 4突变体。此外，ChIP-seq分析表明，CRWN 1直接相互作用的染色质结构域定位在核周边，其中主要包含不可访问的chromatin.Conclusions：综上所述，我们得出结论，CRWN 1是一个关键组成部分的核纤层染色质网络在植物中。它在功能上等同于动物核纤层蛋白，在调节核周围染色质定位模式中发挥关键作用。
Background: The nuclear envelope not only serves as a physical barrier separating nuclear content from the cytoplasm but also plays critical roles in modulating the three-dimensional organization of genomic DNA. For both plants and animals, the nuclear periphery is a functional compartment enriched with heterochromatin. To date, how plants manage to selectively tether chromatin at the nuclear periphery is unclear.Results: By conducting dual-color fluorescence in situ hybridization experiments on 2C nuclei, we show that in Arabidopsis thaliana, specific chromatin positioning at the nuclear periphery requires plant lamin-like proteins CROWDED NUCLEI 1 (CRWN1), CRWN4, and DNA methylation in CHG and CHH contexts. With chromosome painting and Hi-C analyses, we show global attenuation of spatial chromatin compartmentalization and chromatin positioning patterns at the nuclear periphery in both the crwn1 and crwn4 mutants. Furthermore, ChIP-seq analysis indicates that CRWN1 directly interacts with chromatin domains localized at the nuclear periphery, which mainly contains non-accessible chromatin.Conclusions: In summary, we conclude that CRWN1 is a key component of the lamina-chromatin network in plants. It is functionally equivalent to animal lamins, playing critical roles in modulating patterns of chromatin positioning at the nuclear periphery.