A novel brain gene, norbin, induced by treatment of tetraethylammonium in rat hippocampal slice and accompanied with neurite-outgrowth in neuro 2a cells
A novel brain gene, norbin, induced by treatment of tetraethylammonium in rat hippocampal slice and accompanied with neurite-outgrowth in neuro 2a cells
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DOI：
10.1006/bbrc.1997.7660
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发表时间：
1997-11-26
影响因子：
3.1
通讯作者：
Obata, K
中科院分区：
文献类型：
--
作者：
Shinozaki, K;Maruyama, K;Obata, K
四乙基铵（TEA）诱导大鼠海马切片长时程增强（LTP）样突触增强。为了找到与这一现象相关的基因，我们将tea处理的脑组织切片与未处理的全脑切片的mRNA进行减法筛选。其中一个由TEA诱导的克隆，命名为norbin，仅在神经组织中表达。预测的norbin蛋白序列由729个氨基酸组成，与已知基因或蛋白无同源性。转染cDNA诱导培养的neur2a细胞过表达去甲素诱导神经突生长。由于在神经可塑性过程中会发生新突触的形成，神经突生长相关蛋白norbin可能在神经可塑性中起重要作用。(C) 1997学术出版社。
Tetraethylammonium (TEA) induces long-term potentiation (LTP)-like synaptic enhancement in rat hippocampal slices. To find the genes related to this phenomenon, subtraction screening was performed between the mRNA of TEA-treated slices and that of untreated whole brain. One of the clones induced by the TEA treatment, named as norbin, was expressed only in neural tissues. The predicted protein sequence of norbin consisted of 729 amino acids, and no homologies in the sequence were found with known genes or proteins. Overexpression of norbin in cultured Neuro 2a cells by cDNA transfection induced neurite outgrowth. Since in the course of neural plasticity the formation of new synapses should occur, the neurite-outgrowth-related protein, norbin, might play an important role in neural plasticity. (C) 1997 Academic Press.