Multiplex Flow Cytometric Immunoassays for High-Throughput Screening of Multiple Mycotoxin Residues in Milk
Multiplex Flow Cytometric Immunoassays for High-Throughput Screening of Multiple Mycotoxin Residues in Milk
复制标题
用于高通量筛选牛奶中多种霉菌毒素残留的多重流式细胞免疫分析
DOI：
10.1007/s12161-018-01412-4
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发表时间：
2019-04-01
影响因子：
2.9
通讯作者：
Jiang, Wenxiao
中科院分区：
文献类型：
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作者：
Qu, Jianwen;Xie, Huijuan;Jiang, Wenxiao
包括流式细胞术在内的基于微球的免疫分析由于其简单的分析格式和多路分析能力，最近在蛋白质检测和传染病诊断中得到了广泛的应用。本研究选用黄曲霉毒素M1（AFM1）、赭曲霉毒素A （OTA）、脱氧雪腐镰刀菌醇（DON）和伏马毒素B1（FB1）四种真菌毒素作为模型分析物。建立了一种检测牛奶中多种霉菌毒素残留的四重流式细胞免疫分析法（FCIA）。优化后的四联FCIA与多种霉菌毒素残留分析具有良好的相容性，AFM1的检出限为0.045 μ L−1，OTA的检出限为0.94 μ L−1，DON的检出限为7.48 μ L−1，FB1的检出限为2.45 μ L−1。加标牛奶中目标霉菌毒素的回收率为76 ~ 95%，相对标准偏差小于13.4%。与传统ELISA法相比，FCIA法对多种霉菌毒素的检出限较低。
Microsphere-based immunoassays involving flow cytometry have recently gained popularity for use in protein detection and infectious disease diagnosis due to their simple assay format and capacity for multiplexed analysis. In this work, four mycotoxins, including aflatoxin M1(AFM1), ochratoxin A (OTA), deoxynivalenol (DON), and fumonisin B1(FB1), were selected as model analytes. A quadplex flow cytometric immunoassay (FCIA) was developed for detecting multiple mycotoxin residues in milk. The optimized quadplex FCIA exhibited satisfactory compatibility with the multiple mycotoxin residue analysis, with limits of detection (LODs) of 0.045 μg L−1for AFM1, 0.94 μg L−1for OTA, 7.48 μg L−1for DON, and 2.45 μg L−1for FB1. The recoveries of the target mycotoxins from spiked milk were 76–95%, with a relative standard deviation of less than 13.4%. Compared to traditional ELISA formats, the FCIA provided lower detection limits for multiple mycotoxin detection.