Decoding of Quantum Dots Encoded Microbeads Using a Hyperspectral Fluorescence Imaging Method
Decoding of Quantum Dots Encoded Microbeads Using a Hyperspectral Fluorescence Imaging Method
复制标题
使用高光谱荧光成像方法解码量子点编码微珠
DOI：
10.1021/acs.analchem.5b00398
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发表时间：
2015-05-19
影响因子：
7.4
通讯作者：
Ma, Hui
中科院分区：
文献类型：
--
作者：
Liu, Yixi;Liu, Le;Ma, Hui
提出了一种利用线扫描高光谱荧光成像（HFI）技术对量子点编码微珠及其荧光光谱进行解码的方法。提出了一种HFI方法，可以同时获得荧光信号的光谱和编码微珠的空间信息。采用译码方案对HFI系统采集的多色微珠光谱进行译码。对HFI系统与流式细胞仪进行了对比实验。结果表明，HFI系统具有较高的频谱分辨率；因此，可以使用更多的频谱维度通道。用固定化的单链DNA （ssDNA）多色珠进行了HFI系统的检测和解码实验，结果表明了HFI系统的有效性。用扫描电镜观察了聚多巴胺对微球的表面修饰，用激光共聚焦显微镜观察了ssDNA的固定。这些结果表明，所设计的HFI系统可以应用于实际的生物和医学应用。
We presented a decoding method of quantum dots encoded Microbeads with its fluorescence spectra using line scan hyperspectral fluorescence imaging (HFI) method. A HFI method was developed to attain both the spectra of fluorescence signal and the spatial information of the encoded microbeads. A decoding scheme was adopted to decode the spectra of multicolor microbeads acquired by the HFI System. Comparison experiments between the HFI system and the flow cytometer were Conducted. The results showed that the HFI System has higher spectrum resolution; thus, more channels in spectral dimension can be used. The HFI system detection and decoding experiment with the single-stranded DNA (ssDNA) immobilized multicolor beads was,done, and the result showed the efficiency of the HFI system. Surface modification: of the microbeads by use of the polydopamine was characterized by the scanning electron microscopy and ssDNA immobilization was characterized by the :laser confocal microscope. These results indicate that the designed HFI system can be applied to practical biological and medical applications.