DEVELOPMENTALLY REGULATED FETAL THYMIC AND EXTRATHYMIC T-CELL RECEPTOR-GAMMA-DELTA GENE-EXPRESSION
DEVELOPMENTALLY REGULATED FETAL THYMIC AND EXTRATHYMIC T-CELL RECEPTOR-GAMMA-DELTA GENE-EXPRESSION
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DOI：
10.1101/gad.4.8.1304
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发表时间：
1990-08-01
影响因子：
10.5
通讯作者：
HAYDAY, AC
中科院分区：
文献类型：
--
作者：
CARDING, SR;KYES, S;HAYDAY, AC
γ..δ的T细胞受体（TCR）是第一个在所有脊椎动物中个体发育中表达的TCR，其在脊椎动物中已被彻底研究。鼠γδ的在妊娠第14天检测到细胞表面蛋白。在这项工作中，γ的激活。δ的RNA已被研究。数据表明胸腺中出现的第一个TCR蛋白由γ编码。这些基因在细胞进入胸腺后被激活。然而，不同的γ的顺序出现。δ的胸腺个体发育期间的TCR蛋白不能容易地用V γ的不同时间激活来解释。胸腺中的基因。存在不同的γ模式。和δ胎儿肝脏发育期间和胎儿肠道（或与其相关的组织）中的基因表达。出生时小鼠肝脏中明显的细胞表达γ。δ的细胞表面蛋白质，但它们很快就会从肝脏中消失。一个V γ基因重排并在胸腺前表达。此外，γ在新生无胸腺小鼠的肝脏中可检测到基因表达。总之，这些观察结果表明TCR基因的激活的胸腺非依赖性途径。
The .gamma..delta. T-cell receptor (TCR) is the first TCR to be expressed in ontogeny in all vertebrates in which it has been examined thoroughly. Murine .gamma..delta. cell-surface protein is detected by the fourteenth day of gestation. In this work, the activation of .gamma..delta. RNA has been studied. Data indicate that the first TCR protein to appear in the thymus is encoded by .gamma. genes that are activated after cells colonize the thymus. However, the sequential appearance of different .gamma..delta. TCR proteins during thymic ontogeny cannot be readily explained by differential temporal activation of V.gamma. genes in the thymus. There are distinct patterns of .gamma. and .delta. gene expression during fetal liver development and in the fetal gut (or tissue associated with it). Cells apparent in the liver of mice at birth express .gamma..delta. cell-surface protein, but they disappear from the liver very soon afterward. One V.gamma. gene is rearranged and expressed prethymically. In addition, .gamma. gene expression is detectable in the livers of newborn athymic mice. Together, these observations indicate a thymic-independent pathway of activation of TCR genes.