Cellular projections from sensory hair cells form polarity-specific scaffolds during synaptogenesis.
Cellular projections from sensory hair cells form polarity-specific scaffolds during synaptogenesis.
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DOI：
10.1101/gad.259838.115
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发表时间：
2015-05-15
影响因子：
10.5
通讯作者：
Hudspeth AJ
中科院分区：
文献类型：
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作者：
Dow E;Siletti K;Hudspeth AJ
利用荧光共聚焦显微镜的延时成像和连续的块面电子显微镜的互补组合，Dow等人。发现了一种参与脊椎动物神经系统组装的新型突触前投射。神经系统的组装需要轴突和树突延伸到特定的区域，在那里它们与适当的突触靶点相匹配。尽管引导长程生长的线索已经被广泛描述，但还需要额外的机制来解释神经发育中的短程指导。利用荧光共聚焦显微镜的延时成像和连续的块面电子显微镜的互补组合，我们识别了一种参与脊椎动物神经系统组装的新型突触前投射。斑马鱼侧线的每个毛细胞在细胞出生后的特定时间段形成突触。在同一时期，投射从细胞体产生，延伸到成熟毛细胞的特定亚群，并与极性特定的传入神经末梢相互作用。然后，终末沿着投射延伸到适当匹配的突触前位置，之后投射消退。我们的结果表明，突触前投射作为短程伴侣匹配的瞬时支架，这一机制可能发生在神经系统的其他地方。
Using a complementary combination of time-lapse imaging by fluorescence confocal microscopy and serial block-face electron microscopy, Dow et al. identified a novel type of presynaptic projection that participates in the assembly of the vertebrate nervous system. The assembly of a nervous system requires the extension of axons and dendrites to specific regions where they are matched with appropriate synaptic targets. Although the cues that guide long-range outgrowth have been characterized extensively, additional mechanisms are required to explain short-range guidance in neural development. Using a complementary combination of time-lapse imaging by fluorescence confocal microscopy and serial block-face electron microscopy, we identified a novel type of presynaptic projection that participates in the assembly of the vertebrate nervous system. Synapse formation by each hair cell of the zebrafish's lateral line occurs during a particular interval after the cell's birth. During the same period, projections emerge from the cellular soma, extending toward a specific subpopulation of mature hair cells and interacting with polarity-specific afferent nerve terminals. The terminals then extend along the projections to reach appropriately matched presynaptic sites, after which the projections recede. Our results suggest that presynaptic projections act as transient scaffolds for short-range partner matching, a mechanism that may occur elsewhere in the nervous system.
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DOI：
10.1073/pnas.0912082106
发表时间：
2009-12-22
影响因子：
11.1
作者：
Nagiel, Aaron;Patel, Suchit H.;Hudspeth, A. J.
通讯作者：
Hudspeth, A. J.
影响因子：
3
作者：
West, Megan C.;McDermott, Brian M., Jr.
通讯作者：
McDermott, Brian M., Jr.
影响因子：
4.6
作者：
Wibowo, Indra;Pinto-Teixeira, Filipe;Lopez-Schier, Hernan
通讯作者：
Lopez-Schier, Hernan
影响因子：
--
作者：
Kawakami K;Abe G;Asada T;Asakawa K;Fukuda R;Ito A;Lal P;Mouri N;Muto A;Suster ML;Takakubo H;Urasaki A;Wada H;Yoshida M
通讯作者：
Yoshida M
影响因子：
64.8
作者：
通讯作者：
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