Peroxidase-Like Activity of Ethylene Diamine Tetraacetic Acid and Its Application for Ultrasensitive Detection of Tumor Biomarkers and Circular Tumor Cells
Peroxidase-Like Activity of Ethylene Diamine Tetraacetic Acid and Its Application for Ultrasensitive Detection of Tumor Biomarkers and Circular Tumor Cells
复制标题
乙二胺四乙酸的类过氧化物酶活性及其在肿瘤生物标志物和圆形肿瘤细胞超灵敏检测中的应用
DOI：
10.1021/acs.analchem.6b02966
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发表时间：
2017-01-03
影响因子：
7.4
通讯作者：
Liu, Xuanyong
中科院分区：
文献类型：
--
作者：
Huang, Haowen;Liu, Lanfang;Liu, Xuanyong
乙二胺四乙酸（EDTA）是一种功能强大的螯合剂，它可以与大多数金属离子形成稳定的络合物，在工业、农业、环境和医药技术中有着广泛的应用。最近，EDTA被发现可以增强一些材料的光催化性能。受此启发，我们进一步研究了EDTA的光催化性能，发现其与天然辣根过氧化物酶（HRP）的光催化性能基本相同。这一重要的发现可能会扩展传统EDTA在生命科学中的应用。设计了一种基于EDTA类过氧化物酶和独特的金纳米棒（GNRs）的新型比色传感器。在光照下，EDTA可以催化过氧化氢的分解，并原位调节GNR的纵向等离子体波长（LPW），显示各种颜色的溶液作为读出手段。这种比色纳米传感器具有很大的潜力，发展成为一个平台，以定量测定的aualytes，只要针对他们的特异性抗体是可用的。不同疾病的生物标志物，如乳腺癌和前列腺癌，被检测出具有高准确性。此外，结合免疫磁性分离血液中循环肿瘤细胞（CTC），建立了CTC的可视化检测方法，在临床诊断、环境监测和食品质量控制等领域具有广阔的应用前景。
Ethylene diamine tetraacetic acid (EDTA) is such a powerful chelating agent that it may form stable complexes with most metal ions, which has wide applications in industry, agriculture, environment, and pharmaceutical technology. Recently, EDTA was found to enhance the photocatalytic property of some materials. Inspired by this fact of EDTA in the photocatalytic role, we further investigated the photocatalytic property of EDTA and found much the same as that of natural horseradish peroxidase (HRP). This significant discovery of peroxidase-like property may extend the applications of conventional EDTA in life science. A novel and colorimetric sensor based on the peroxidase-like EDTA and unique gold nanorods (GNRs) was designed. Under light irradiation, EDTA may catalyze decomposition of hydrogen peroxide and in situ regulate the longitudinal plasmon wavelength (LPW) of GNRs, displaying various color solution as a read-out means. This colorimetric nanosensor has a great potential to develop into a platform to quantitatively determine aualytes as long as the specific antibodies against them were available. Biomarkers of different diseases, such as breast cancer and prostate cancer, were detected with high accuracy. Moreover, combined with immunomagnetic separation of circulating tumor cells (CTCs) from blood, a visual read-out for detection of CTCs was established, which has promising applications in clinical diagnosis, environmental monitoring, and food quality control only using naked eyes.