Arabidopsis EARLY FLOWERING3 increases salt tolerance by suppressing salt stress response pathways
Arabidopsis EARLY FLOWERING3 increases salt tolerance by suppressing salt stress response pathways
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DOI：
10.1111/tpj.13747
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发表时间：
2017-12-01
期刊：
影响因子：
7.2
通讯作者：
Paek, Nam-Chon
中科院分区：
文献类型：
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作者：
Sakuraba, Yasuhito;Bulbul, Selin;Paek, Nam-Chon
拟南芥EARLY FLOWERING 3（ELF 3）是ELF 3-ELF 4-LUX ARRHYTHMO（LUX）夜间复合体的一个组成部分，参与调节光对生物钟的作用。然而，ELF 3在应激反应中的作用在很大程度上仍然未知。在这项研究中，我们发现ELF 3增强了植物对盐胁迫的适应性：ELF 3过表达（ELF 3-OX）植物是耐盐的，而elf 3突变体对盐胁迫更敏感。与野生型相比，许多盐胁迫和衰老相关基因的表达在elf 3 -1和ELF 3 OX植物中发生了改变。在盐胁迫期间，ELF 3在翻译后水平抑制促进盐胁迫响应途径的因子，主要是巨蛋白（GI），在转录水平抑制植物色素相互作用因子4（PIF 4）。为了增强盐胁迫响应，PIF 4直接下调JUNGBRUNNEN 1（JUB 1/ANAC 042）的转录，JUNGBRUNNEN 1编码上调胁迫耐受基因DREB 2A和DELLA表达的转录因子。此外，PIF 4直接上调ORESARA 1（ORE 1/ANAC 092）和SAG 29的转录，这两种基因是盐胁迫反应途径的正调节因子。基于我们的研究结果，我们建议，ELF 3调制盐胁迫反应途径中的关键调控成分在转录和翻译后水平。
Arabidopsis EARLY FLOWERING3 (ELF3) functions in modulating light input to the circadian clock, as a component of ELF3-ELF4-LUX ARRHYTHMO (LUX) evening complex. However, the role of ELF3 in stress responses remains largely unknown. In this study, we show that ELF3 enhances plants' resilience to salt stress: ELF3-overexpressing (ELF3-OX) plants are salt-tolerant, while elf3 mutants are more sensitive to salt stress. The expressions of many salt stress-and senescence-associated genes are altered in elf3-1 and ELF3OX plants compared with wild-type. During salt stress, ELF3 suppresses factors that promote salt stress response pathways, mainly GIGANTEA (GI), at the post-translational level, and PHYTOCHROME INTERACTING FACTOR4 (PIF4), at the transcriptional level. To enhance the salt stress response, PIF4 directly downregulates the transcription of JUNGBRUNNEN1 (JUB1/ANAC042), encoding a transcription factor that upregulates the expression of stress tolerance genes, DREB2A and DELLA. Furthermore, PIF4 directly upregulates the transcription of ORESARA1 (ORE1/ANAC092) and SAG29, positive regulators of salt stress response pathways. Based on our results, we propose that ELF3 modulates key regulatory components in salt stress response pathways at the transcriptional and post-translational levels.