Biological Uptake and Depuration of Radio-labeled Graphene by Daphnia magna
Biological Uptake and Depuration of Radio-labeled Graphene by Daphnia magna
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大型水蚤对放射性标记石墨烯的生物摄取和纯化
DOI：
10.1021/es403230u
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发表时间：
2013-11-05
影响因子：
11.4
通讯作者：
Mao, Liang
中科院分区：
文献类型：
--
作者：
Guo, Xiangke;Dong, Shipeng;Mao, Liang
石墨烯薄膜在大量应用中具有潜在的应用前景。然而，人们对它们的生态毒理风险知之甚少，这主要是因为在复杂的环境基质中缺乏量化技术。本研究采用三明治状FePO4/十二胺杂化纳米片的石墨化和剥离方法合成了石墨烯，并在合成中引入了C-14。将C-14标记的石墨烯添加到人工淡水中，评价大型水蚤对石墨烯的吸收和净化。石墨烯250mg/L溶液作用24 h后，石墨烯在生物体中的浓度接近生物体干重的1%。这些生物排泄石墨烯以清洁人工淡水，并在24小时净化周期后实现大致恒定的身体负荷，而无论初始石墨烯暴露浓度如何。在净化期间向水中添加藻类和腐植酸，可释放大量(>；90%)积累的石墨烯，但仍有一些残留在生物体中。成年水蚤体内积累的石墨烯很可能转移到新生儿体内。本文提供的吸收和消除结果支持石墨烯的环境风险评估，石墨烯定量方法是进一步研究的有力工具。
Graphene layers are potential candidates in a large number of applications. However, little is known about their ecotoxicological risks largely as a result of a lack of quantification techniques in complex environmental matrices. In this study, graphene was synthesized by means of graphitization and exfoliation of sandwich-like FePO4/dodecylamine hybrid nanosheets, and C-14 was incorporated in the synthesis. C-14-labeled graphene was spiked to artificial freshwater and the uptake and depuration of graphene by Daphnia magna were assessed. After exposure for 24 h to a 250 mu g/L solution of graphene, the graphene concentration in the organism was nearly 1% of the organism dry mass. These organisms excreted the graphene to clean artificial freshwater and achieved roughly constant body burdens after 24 h depuration periods regardless of the initial graphene exposure concentration. Addition of algae and humic acid to water during the depuration period resulted in release of a significant fraction (>90%) of the accumulated graphene, but some still remained in the organism. Accumulated graphene in adult Daphnia was likely transferred to the neonates. The uptake and elimination results provided here support the environmental risk assessment of graphene and the graphene quantification method is a powerful tool for additional studies.