Macrophage killing is an essential virulence mechanism of Salmonella typhimurium
Macrophage killing is an essential virulence mechanism of Salmonella typhimurium
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DOI：
10.1073/pnas.93.9.4197
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发表时间：
1996-04-30
影响因子：
11.1
通讯作者：
Heffron, F
中科院分区：
文献类型：
--
作者：
Lindgren, SW;Stojiljkovic, I;Heffron, F
吞噬细胞是抵御感染的关键防线。病原体在吞噬细胞内生存甚至复制的能力是逃避宿主防御机制的有效方法。许多病原体在吞噬作用后在巨噬细胞内存活，这增加了它们的毒力。沙门氏菌就是这些病原体之一。在这里，我们报告沙门氏菌进入巨噬细胞并复制后 6-14 小时，它驻留在大液泡中并导致这些细胞的破坏。此外，我们还鉴定了四种独立分离的 MudJ-lacZ 插入突变体，它们不再导致这些空泡的形成或杀死巨噬细胞。所有四个插入均位于 ompR/envZ 调节子中。这些发现表明，巨噬细胞的杀死和逃逸可能与这些宿主细胞中的存活和复制一样，是沙门氏菌发病机制中的重要步骤。
Phagocytic cells are a critical Line of defense against infection. The ability of a pathogen to survive and even replicate within phagocytic cells is a potent method of evading the defense mechanisms of the host. A number of pathogens survive within macrophages after phagocytosis and this contributes to their virulence. Salmonella is one of these pathogens. Here we report that 6-14 hr after Salmonella enters the macrophage and replicates, it resides in large vacuoles and causes the destruction of these cells. Furthermore, we identified four independently isolated MudJ-lacZ insertion mutants that no longer cause the formation of these vacuoles or kill the macrophages. All four insertions were located in the ompR/envZ regulon. These findings suggest that killing and escape from macrophages may be as important steps in Salmonella pathogenesis as are survival and replication in these host cells.