Synthesis and photophysical properties of a new push-pull pyrene dye with green-to-far-red emission and its application to human cellular and skin tissue imaging
Synthesis and photophysical properties of a new push-pull pyrene dye with green-to-far-red emission and its application to human cellular and skin tissue imaging
复制标题
DOI：
10.1039/d1tb02728j
复制
发表时间：
2022-02-14
影响因子：
7
通讯作者：
Niko, Yosuke
中科院分区：
文献类型：
--
作者：
Inoue, Kazuki;Kawakami, Ryosuke;Niko, Yosuke
在此，我们讨论了一种新的芘基推拉染料（PC）和我们的调查，其物理性质和适用性的生物研究。新合成的染料在相当宽的范围内（即，绿色至远红区域），伴随着高荧光量子产率（在大多数有机溶剂中Phi（FL）> 0.70）和上级常用尼罗红（NR）染料的光稳定性，后者也在绿色至红色区域发荧光。当用PC染色的人前列腺癌细胞使用共聚焦激光扫描荧光显微镜成像时，发现PC选择性地染色脂滴。在液滴形成受到抑制的细胞条件下，PC可以分布到剩余的液滴和细胞间膜，这可以基于PC的荧光溶致变色功能来区分。此外，PC有效地染色正常人皮肤组织块处理的增强剂，并使每个组织结构中的单个细胞通过双光子荧光显微镜（2PM）的清晰可视化。有趣的是，PC在组织穿透的960 nm激发下提供明亮的2PM图像，实现了比传统芘染料和NR更清晰和更深的组织成像。这些结果表明，PC可以取代几种常用的染料在各种生物应用，特别是快速和准确的诊断组织疾病，活检为代表。
Herein, we discuss a new pyrene-based push-pull dye (PC) and our investigation of its photophysical properties and applicability to biological studies. The newly synthesized dye exhibits highly polarity-sensitive fluorescence over a significantly wide range (i.e., the green to far-red region), accompanied by high fluorescence quantum yields (Phi(FL) > 0.70 in most organic solvents) and superior photostability to that of the commonly used Nile Red (NR) dye, which also fluoresces in the green to red region. When human prostate cancer cells stained with PC were imaged using a confocal laser scanning fluorescence microscope, PC was found to selectively stain the lipid droplets. Under the cell conditions where the formation of droplets was inhibited, PC could be distributed to both the remaining droplets and the intercellular membranes, which could be distinguished based on the fluorescence solvatochromic function of PC. Furthermore, PC efficiently stained normal human skin tissue blocks treated with a transparency-enhancing agent and enabled clear visualization of individual cells in each tissue architecture by means of two-photon fluorescence microscopy (2PM). Interestingly, PC provides bright 2PM images under tissue-penetrative 960 nm excitation, realizing much clearer and deeper tissue imaging than conventional pyrene dyes and NR. These results suggest that PC could replace several commonly used dyes in various biological applications, particularly the rapid and accurate diagnosis of tissue diseases, typified by biopsy.