Studies on the expression of the microtubule-associated protein, tau, during mouse brain development, with newly isolated complementary DNA probes.
Studies on the expression of the microtubule-associated protein, tau, during mouse brain development, with newly isolated complementary DNA probes.
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DOI：
10.1083/jcb.98.3.1090
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发表时间：
1984-03
期刊：
影响因子：
--
通讯作者：
Kirschner MW
中科院分区：
文献类型：
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作者：
Drubin DG;Caput D;Kirschner MW
微管相关蛋白Tau是一组在体内与微管相关联且在体外能刺激微管组装的密切相关的多肽。我们使用一种针对牛脑微管相关蛋白Tau的亲和纯化抗血清发现，在小鼠大脑发育过程中，Tau多肽的数量和含量发生了巨大变化。由于体外翻译产物反映了体内发现的质和量的变化，所以不同的形式似乎是由Tau mRNA的变化导致的。为了研究Tau蛋白的mRNA和基因组复杂性，我们使用从多聚核糖体中用Tau抗血清纯化的Tau mRNA来分离胚胎小鼠Tau互补DNA克隆。利用这些探针，我们已经确定胚胎Tau蛋白是由一种在整个大脑发育过程中都存在的6 - kb的mRNA翻译而来的。
Tau protein is a collection of closely related polypeptides that associate with microtubules in vivo and stimulate their assembly in vitro. Using an affinity-purified antiserum against bovine brain tau protein, we found that the number and amount of tau polypeptides changes dramatically during mouse brain development. The different forms appear to result from changes in tau mRNA since in vitro translation products reflect the qualitative and quantitative changes found in vivo. To study the mRNA and genomic complexity of tau protein, we used tau mRNA, purified from polysomes with tau antiserum, to isolate embryonic mouse tau complementary DNA clones. With these probes we have determined that embryonic tau protein is translated from a 6-kb mRNA that persists throughout brain development.