Effect of colchicine binding on the reversible dissociation of the tubulin dimer.
Effect of colchicine binding on the reversible dissociation of the tubulin dimer.
复制标题
秋水仙碱结合对微管蛋白二聚体可逆解离的影响。
DOI：
10.1021/bi00539a018
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发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Wilson,L
中科院分区：
文献类型：
--
作者：
Detrich3rd,HW;WilliamsJr,RC;Wilson,L
材料与方法秋水仙碱（CLC）、1 Pipes、EGTA、DTE和GTP（II-S型）从Sigma Chemical Co.获得。鬼臼毒素（PLN）和托酚酮（TRO）是Aldrich Chemical Co.的产品。秋水仙碱（环C，甲氧基-3H），16.05 Ci/mmol，购自新英格兰核能公司。实验条件。所有实验均在PM缓冲液[0.1 M Pipes-NaOH（pH 6.9）、2 mM EGTA、1 mM MgS 〇 4、0.1 mM GTP和2 mM DTE）中进行，并添加所示的药物。该缓冲液的离子强度约为0.236，在4.95 ℃时其密度为1.017 g/mL（Detrich &威廉姆斯，1978）.温度在下面详细说明。
Materials and MethodsReagents. Colchicine (CLC), 1 Pipes, EGTA, DTE, and GTP (type II-S) were obtained from Sigma Chemical Co. Podophyllotoxin (PLN) and tropolone (TRO) were products of Aldrich Chemical Co. Colchicine (ring C, methoxy-3H), 16.05 Ci/mmol, was purchased from New England Nuclear, Inc.Experimental Conditions. All experiments were performed in PM buffer [0.1 M Pipes-NaOH (pH 6.9), 2 mM EGTA, 1 mM MgS04, 0.1 mM GTP, and 2 mM DTE) with the addition of drugs as indicated. The ionic strength of this buffer was approximately 0.236, and its density was 1.017 g/mL at 4.95 C (Detrich & Williams, 1978). Temperatures are de-tailed below.