STRUCTURE, CELL-SPECIFIC EXPRESSION, AND MATING-INDUCED REGULATION OF A DROSOPHILA-MELANOGASTER MALE ACCESSORY-GLAND GENE
STRUCTURE, CELL-SPECIFIC EXPRESSION, AND MATING-INDUCED REGULATION OF A DROSOPHILA-MELANOGASTER MALE ACCESSORY-GLAND GENE
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DOI：
10.1016/0012-1606(90)90284-p
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发表时间：
1990-05-01
影响因子：
2.7
通讯作者：
WOLFNER, MF
中科院分区：
文献类型：
--
作者：
DIBENEDETTO, AJ;HARADA, HA;WOLFNER, MF
雄性昆虫的附腺是生殖道的分泌组织，由几种不同的细胞类型组成。它分泌射精液的成分，这些成分对雌性交配后的行为有重要影响。我们已经研究了MST 316基因的序列、结构和表达，该基因仅在雄性黑腹果蝇的副腺中表达。MST 316RNA编码一个由52个氨基酸组成的小的碱性蛋白质，它具有分泌肽前体的共同特征，包括一个疏水的N末端。通过胚系转化将MST 316-lacZ杂合基因导入果蝇体内，研究了MST 316基因的组织特异性表达。MST 316-LacZ融合蛋白仅在附腺的“主”细胞中表达。它首先在羽化时被检测到，并在成虫生命的头3天表现出突然的合成。交配刺激融合蛋白的合成，因此允许与雌性交配的雄性与相同年龄的处女对照相比，β-半乳糖苷酶活性水平高出两到六倍。交配刺激的MST 316-LacZ融合蛋白的合成，以及根据天然基因产物的推断，似乎至少部分是由于转录水平的增加。
The accessory gland of male insects is a secretory tissue of the genital tract made up of several distinct cell types. It secretes components of the ejaculatory fluid which have an important effect on the postmating behavior of the female. We have examined the sequence, structure, and expression of a gene, mst 316, expressed exclusively in the accessory glands of male Drosophila melanogaster. The mst 316 RNA encodes a small, basic protein of 52 amino acids that exhibits features common to precursors of secreted peptides, including a hydrophobic N-terminus. The tissue-specific expression of the mst 316 gene was studied using an mst 316-lacZ hybrid gene inserted into Drosophila by germ line transformation. The mst 316-lacZ fusion protein is expressed exclusively in the "main" cells of the accessory gland. It is first detected upon eclosion and exhibits a burst of synthesis in the first 3 days of adult life. The synthesis of the fusion protein is stimulated by mating, so that .beta.-galactosidase activity levels are two- to sixfold higher in males allowed to copulate with females compared to virgin male controls of the same age. The mating-stimulated synthesis of the mst 316-lacZ fusion protein, and by inference of the native gene product, appears to be due at least in part to increased transcript levels.