Binding of the differentiation-inducer, granulocyte-colony-stimulating factor, to responsive but not unresponsive leukemic cell lines.
Binding of the differentiation-inducer, granulocyte-colony-stimulating factor, to responsive but not unresponsive leukemic cell lines.
复制标题
分化诱导剂、粒细胞集落刺激因子与有反应但并非无反应的白血病细胞系结合。
DOI：
10.1073/pnas.81.12.3765
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发表时间：
1984
影响因子：
11.1
通讯作者：
Metcalf,D
中科院分区：
文献类型：
--
作者：
Nicola,NA;Metcalf,D
粒细胞集落刺激因子（G-CSF）是一种组织来源的25000 Mr糖蛋白，在体外刺激小鼠骨髓祖细胞形成中性粒细胞集落。它也是小鼠髓单细胞白血病细胞系WEHI-3B的终末分化和干细胞更新的有效诱导剂。纯化后的G-CSF经放射性碘化处理后具有高比放射性，并保留了充分的生物活性。碘化G-CSF特异性结合WEHI-3B细胞、J774巨噬细胞肿瘤细胞和正常小鼠骨髓细胞，但不结合其他多种肿瘤细胞系或小鼠胸腺细胞。WEHI-3B细胞对125i标记的G-CSF表现出高亲和力（Kd = 90 pM），但在37℃时仅显示少量特异性受体（每个细胞300-700个）。其他纯化的集落刺激因子对这些受体的结合没有竞争。WEHI-3B （D-）是一种不能被G-CSF诱导分化的WEHI-3B亚系，未与该因子特异性结合，提示其受体呈递不足。
Granulocyte-colony-stimulating factor (G-CSF) is a tissue-derived 25,000 Mr glycoprotein that stimulates neutrophilic granulocyte colony formation from murine bone marrow progenitor cells in vitro. It is also a potent inducer of terminal differentiation and suppressor of stem cell renewal in the murine myelomonocytic leukemic cell line WEHI-3B. Purified G-CSF was radioiodinated to high specific radioactivity with retention of full biological activity. Iodinated G-CSF bound specifically to WEHI-3B cells, J774 macrophage tumor cells, and normal murine bone marrow cells but not to a variety of other tumor cell lines or murine thymocytes. WEHI-3B cells showed a high affinity for 125I-labeled G-CSF (Kd = 90 pM) but displayed only a small number of specific receptors (300-700 per cell) at 37 degrees C. Other purified colony-stimulating factors showed no competition for binding to these receptors. WEHI-3B (D-), a subline of WEHI-3B that cannot be induced to differentiate by G-CSF, showed no specific binding of this factor, indicating that it is deficient in receptor presentation.