Endoplasmic Reticulum Stress Signals in Defined Human Embryonic Stem Cell Lines and Culture Conditions
Endoplasmic Reticulum Stress Signals in Defined Human Embryonic Stem Cell Lines and Culture Conditions
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DOI：
10.1007/s12015-010-9135-4
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发表时间：
2010-09-01
影响因子：
4.8
通讯作者：
Lopez-Larrea, Carlos
中科院分区：
文献类型：
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作者：
Angel Blanco-Gelaz, Miguel;Suarez-Alvarez, Beatriz;Lopez-Larrea, Carlos
人胚胎干细胞（hESC）对几种细胞应激具有特别的抵抗力，但内质网（ER）应激的存在和培养条件的诱导尚不清楚。使用qPCR，在这里，我们研究了ER应激的主要传感器的行为及其与饲养层、无饲养系统中使用的条件培养基的类型和几种分化标志物的上调的关系。我们观察了HS 181和SHEF 1 hESCs在不同条件培养液（HEF-CM和ASC-CM）作用下在脂肪间充质干细胞（ASC）和无饲养层培养系统中的多能性保持情况，并检测了分化标志物的差异表达。总之，这些结果证明了ER应激事件的证据，细胞必须解决这些事件才能存活并维持多能性标志物。所定义的早期分化状态可以进展到更分化的状态，并且可能受培养条件的影响。
Human embryonic stem cells (hESCs) are especially resistant to several cellular stresses, but the existence and induction of Endoplasmic Reticulum (ER) stress by culture conditions are unknown. Using qPCR, here, we investigated the behavior of the principal sensors of ER stress and their relation with the feeder layer, the type of conditioned media used in feeder free systems and the upregulation of several differentiation markers. We observed the preservation of pluripotency, and detected differential expression of differentiation markers in HS 181 and SHEF1 hESCs growing on Adipose-derived mesenchymal stem cells (ASCs) and feeder-free system with different conditioned media (HEF-CM and ASC-CM). Taken together, these results demonstrate evidence of ER stress events that cells must resolve to survive and maintenance of markers of pluripotency. The early differentiation status defined could progress into a more differentiated state, and may be influenced by culture conditions.