Structure and composition of biofilm communities in a moving bed biofilm reactor for nitritation-anammox at low temperatures
Structure and composition of biofilm communities in a moving bed biofilm reactor for nitritation-anammox at low temperatures
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DOI：
10.1016/j.biortech.2013.12.062
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发表时间：
2014-02-01
影响因子：
11.4
通讯作者：
Wilen, Britt-Marie
中科院分区：
文献类型：
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作者：
Persson, Frank;Sultana, Razia;Wilen, Britt-Marie
将厌氧氨氧化（anammox）应用于低温废水脱氮是一项挑战。维持厌氧氨氧化菌和好氧氨氧化细菌（AOB）以及抑制亚硝酸盐氧化细菌（NOB）是关键问题。在这项研究中，一个亚硝化 - 厌氧氨氧化移动床生物膜中试反应器在19 - 10℃下运行了300天。在19 - 13℃时，脱氮量虽在下降，但较为稳定；在10℃时，脱氮变得不稳定。定量PCR、荧光原位杂交和基因测序表明，随着温度降低，未观察到微生物群落发生重大变化。厌氧氨氧化菌在生物膜中占主导地位（每平方米有0.9 - 1.2×10¹⁴个16S rRNA拷贝）。大多数厌氧氨氧化菌与Brocadia sp. 40相似，但在生物膜 - 水界面附近存在另一个较小的Brocadia种群，AOB群落（亚硝化单胞菌）也集中在薄层中（每平方米有1.8 - 5.3×10¹²个amoA拷贝）。NOB（硝化杆菌、硝化螺菌）始终以低浓度存在（……此处原文似乎不完整）
It is a challenge to apply anaerobic ammonium oxidation (anammox) for nitrogen removal from waste-water at low temperatures. Maintenance of anammox- and aerobic ammonia oxidizing bacteria (AOB) and suppression of nitrite oxidizing bacteria (NOB) are key issues. In this work, a nitritation-anammox moving bed biofilm pilot reactor was operated at 19-10 degrees C for 300 d. Nitrogen removal was decreasing, but stable, at 19-13 degrees C. At 10 degrees C removal became unstable. Quantitative PCR, fluorescence in situ hybridization and gene sequencing showed that no major microbial community changes were observed with decreased temperature. Anammox bacteria dominated the biofilm (0.9-1.2 x 10(14) 16S rRNA copies m (2)). Most anammox bacteria were similar to Brocadia sp. 40, but another smaller Brocadia population was present near the biofilm-water interface, where also the AOB community (Nitrosomonas) was concentrated in thin layers (1.8-5.3 10(12) amoA copies m (2)). NOB (Nitrobacter, Nitrospira) were always present at low concentrations (