Corynebacterium glutamicum CgynfM encodes a dicarboxylate transporter applicable to succinate production
Corynebacterium glutamicum CgynfM encodes a dicarboxylate transporter applicable to succinate production
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DOI：
10.1016/j.jbiosc.2018.10.004
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发表时间：
2019-04-01
影响因子：
2.8
通讯作者：
Abe, Keietsu
中科院分区：
文献类型：
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作者：
Fukui, Keita;Nanatani, Kei;Abe, Keietsu
谷氨酸棒杆菌在去除琥珀酸脱氢酶基因后，可以在好氧条件下产生琥珀酸和相当数量的乙酸丙酮酸作为葡萄糖代谢的副产物。最近，我们在Ananatis Pantoea Ananatis(PaynJM)中发现了一个编码二羧酸转运蛋白的基因，并在谷氨酸转运蛋白中发现了一个同源基因(CgynfM)。在这项研究中，我们研究了表达CgynJM的谷氨酸杆菌产生二羧酸盐的情况。在好氧条件下培养表达CgynfM基因的谷氨酸杆菌，其糖耗速率显著提高，琥珀酸积累量从66 mM增加到110 mM，丙酮酸和乙酸酯的产生量显著降低。丙酮酸从120 mm减少到6.2 mm，醋酸盐减少到检测不到的水平。CgYnfM使谷氨酸链霉菌4110655AsucE1菌株在厌氧条件下恢复了琥珀酸产酸，缺失了主要琥珀酸输出体(UcE1)基因。在厌氧条件下，CgynJM的表达还使α-酮戊二酸的产量从5.1 mM增加到24 mM。综上所述，这些结果表明来自谷氨酸杆菌的YnfM作为一种二元酸转运体的功能适用于琥珀酸生产。(C)2018年，日本生物技术学会。版权所有。
After deleting the gene encoding succinate dehydrogenase, Corynebacterium glutamicum can produce succinate and a considerable amount of acetate and pyruvate as by-products from glucose metabolism, under aerobic conditions. Recently, we identified ynfM in Pantoea ananatis (PaynJM) as a gene encoding a dicarboxylate transporter and found a homologous gene (CgynfM) in C. glutamicum. In this study, we examined dicarboxylate production using C. glutamicum strains expressing CgynJM. When C. glutamicum expressing the CgynfM gene was cultured under aerobic conditions, the sugar-consumption rate increased significantly, succinate accumulation increased from 66 mM to 110 mM, and pyruvate and acetate co-production decreased significantly. Pyruvate decreased from 120 mM to 6.2 mM, and acetate decreased to undetectable level. CgYnfM restored succinate production under anaerobic conditions in C. glutamicum strain 4110655AsucE1, in which the gene encoding the major succinate exporter (sucE1) was deleted. CgynJM expression also increased alpha-ketoglutarate production from 5.1 mM to 24 mM under anaerobic conditions. Collectively, these results suggest that YnfM from C. glutamicum functions as a dicarboxylate transporter that is applicable to the succinate production. (C) 2018, The Society for Biotechnology, Japan. All rights reserved.