The Autonomic Innervation and Uterine Telocyte Interplay in Leiomyoma Formation
The Autonomic Innervation and Uterine Telocyte Interplay in Leiomyoma Formation
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DOI：
10.1177/0963689719833303
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发表时间：
2019-05-01
影响因子：
3.3
通讯作者：
Gil, Krzysztof
中科院分区：
文献类型：
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作者：
Aleksandrovych, Veronika;Kurnik-Lucka, Magdalena;Gil, Krzysztof
子宫的自主神经支配参与人类和动物的多种病理生理过程。病理状态如子宫腺肌病或盆腔炎通常伴有子宫神经支配的显著改变。在目前的研究中，我们集中在子宫肌瘤的自主神经支配，最近描述的间质细胞，端细胞的鉴定，以及这些结构之间可能的相互作用。在这项工作中，通过c-kit、CD 34和PDGFR α的免疫阳性鉴定子宫端细胞。通过免疫标记神经元标记物：蛋白质基因产物PGP 9.5、诱导型一氧化氮合酶（iNOS）、胆碱乙酰转移酶（ChAT）和酪氨酸羟化酶（TH）来显示神经。子宫肌层组织的大体组织结构已通过常规组织学进行分析。结果表明，子宫肌瘤组织中iNOS和ChAT免疫阳性神经元密度均高于对照组。纤维化灶端细胞密度低于正常肌层。我们的研究结果表明，自主神经支配和端细胞参与子宫肌瘤的微环境不平衡的特点。由于NOS阳性神经在氧化应激调节中起重要作用，它们可能导致端细胞数量的减少，端细胞是平滑肌瘤形成的发病机制中的重要组成部分。
The autonomic innervation of the uterus is involved in multiple pathophysiological processes in both humans and animals. Pathological conditions such as adenomyosis or inflammatory pelvic disease are usually accompanied by significant alterations in uterine innervation. In the current study, we focused on autonomic innervation of uterine fibroids, the identification of recently described interstitial cells, telocytes, and the possible interplay between these structures. In this work, uterine telocytes were identified by immunopositivity for c-kit, CD34, and PDGFR alpha. Nerves were revealed by immunolabeling for neuronal markers: protein gene product PGP 9.5, inducible nitric oxide synthase (iNOS), choline acetyltransferase (ChAT), and tyrosine hydroxylase (TH). The gross organization of myometrial tissue has been analyzed by routine histology. The results demonstrated that the density of iNOS and ChAT-immunopositive neurons in the uterine fibroids was higher than that in the control samples. The density of telocytes in the fibrosis foci was lower than that in the normal myometrium. Our results suggest that autonomic innervation and telocytes are involved in the microenvironment imbalance characteristic of uterine leiomyoma. Since NOS-positive nerves play an important role in oxidative stress modulation, they might lead to a decrease in the number of telocytes, which are crucial components in the pathogenesis of leiomyoma formation.