Crystallographic Image Processing Applications for S-Layers
Crystallographic Image Processing Applications for S-Layers
复制标题
S 层晶体图像处理应用
DOI：
10.1007/978-1-4757-9032-0_2
复制
发表时间：
1993
影响因子：
3.2
通讯作者：
S. Hovmöller
中科院分区：
文献类型：
--
作者：
S. Hovmöller
许多结晶细菌表面层（S 层）的三维结构已通过电子显微镜进行了研究。蛋白质分子由轻元素（主要是 C、O、N 和 H）组成，这些元素的对比度非常低，并且对电子显微镜中的辐射损伤非常敏感，以至于在蛋白质分子被电子束破坏之前不可能观察到其许多细节。有两种方法可以克服这个困境。一种是将蛋白质嵌入染色介质中，这既可以增加对比度，又可以抵抗辐射损伤。另一种方法是在蛋白质分子被完全破坏之前使用非常低的电子剂量拍摄显微照片。然后可以将数百个这些“嘈杂”蛋白质分子的图像平均为一张图像。这两种方法可以单独使用，也可以结合使用。
The three-dimensional structures of many crystalline bacterial surface layers (S-layers) have been studied by electron microscopy. Protein molecules are composed of light elements (mainly C,O,N and H) which give very little contrast and are so sensitive to radiation damage in the electron microscope that it is impossible to observe many details in a protein molecule before it has been destroyed by the electron beam. There are two ways to overcome this dilemma. One is to embed the protein in a staining medium which both increases the contrast and resists radiation damage. The other way is to take micrographs using very low electron doses, before the protein molecules are completely destroyed. Images of hundreds of these “noisy” protein molecules can then be averaged into one image. These two methods can be used individually or in combination with one another.
3.5 A 分辨率投影中的 PhoE 孔蛋白结构。
影响因子：
3
作者：
Jap,BK;Downing,KH;Walian,PJ
通讯作者：
Walian,PJ
影响因子：
5.6
作者：
HENDERSON, R;BALDWIN, JM;DOWNING, KH
通讯作者：
DOWNING, KH