Predicting the radiosensitivity of HPV-negative oropharyngeal squamous cell carcinoma using miR-130b
Predicting the radiosensitivity of HPV-negative oropharyngeal squamous cell carcinoma using miR-130b
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DOI：
10.1080/00016489.2021.1897160
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发表时间：
2021-03-31
影响因子：
1.4
通讯作者：
Sugasawa, Masashi
中科院分区：
文献类型：
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作者：
Inoue, Hitoshi;Hirasaki, Masataka;Sugasawa, Masashi
研究背景人乳头瘤病毒（HPV）阴性的口咽鳞状细胞癌（oropharyngeal squamous cell carcinoma，OPSCC）比HPV阳性的口咽鳞状细胞癌（oropharyngeal squamous cell carcinoma，HPV）表现出更高的放射抵抗率。放射抗性HPV阴性OPSCC是与不利的结果，但验证的预后生物标志物仍然lacked.Aims/ObjectivesThis研究探讨放射抗性HPV阴性OPSCC.Material和methodsThe癌症基因组图谱的生物标志物包括miRNA序列和mRNA序列数据从528 HNSCC肿瘤。其中，我们使用了HPV阴性头颈部鳞状细胞癌的基因表达数据，这些数据可用于辐射的影响，并比较了辐射抗性组和辐射敏感组之间的miRNA序列和mRNA序列数据。我们随后从结果中估计了下游miRNA。最后，我们验证了miRNA相关的结果，放射治疗在我们的临床cases.ResultsInvestigation的miRNA序列显示的表达的miR-130 b作为最大的差异之间的放射敏感性和放射抵抗组。我们随后评估了我们的临床OPSCC病例中miR-130 b的表达。根据受试者工作特征曲线分析，miR-130 b>5.372（低表达）的值与无进展生存期和总生存期显著延长相关（分别为p = 0.006，p = 0.04）。
BackgroundHuman papillomavirus (HPV)-negative oropharyngeal squamous cell carcinoma shows a higher rate of radiation resistance than HPV-positive oropharyngeal squamous cell carcinoma (OPSCC). Radioresistant HPV-negative OPSCC is associated with unfavourable outcomes, but validated prognostic biomarkers remain lacking.Aims/ObjectivesThis study investigated biomarkers for radioresistant HPV-negative OPSCC.Material and methodsThe Cancer Genome Atlas included miRNA sequence and mRNA sequence data from 528 HNSCC tumours. Of these, we used gene expression data for HPV-negative head and neck squamous cell carcinoma for which data were available on the effects of radiation, and compared miRNA sequence and mRNA sequence data between radioresistant and radiosensitive groups. We subsequently estimated downstream miRNA from the results. Finally, we validated miRNAs related to the outcomes of radiotherapy in our clinical cases.ResultsInvestigation of miRNA sequence revealed expression of miR-130b as the greatest difference between radiosensitive and radioresistant groups. We subsequently evaluated miR-130b expression in our clinical OPSCC cases. Values of miR-130b >5.372 (low expression), determined from receiver operating characteristic curve analyses, were associated with significantly longer progression-free survival and overall survival (p = .006, p = .04, respectively).Conclusions and significanceOur results suggest that miR-130b has potential as a biomarker for the radiosensitivity of HPV-negative OPSCC.