Diversity of the Vaginal Microbiome Correlates With Preterm Birth
Diversity of the Vaginal Microbiome Correlates With Preterm Birth
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DOI：
10.1177/1933719113488838
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发表时间：
2014-01-01
影响因子：
2.9
通讯作者：
Giudice, Linda C.
中科院分区：
文献类型：
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作者：
Hyman, Richard W.;Fukushima, Marilyn;Giudice, Linda C.
生殖道感染是早产（PTB）的主要诱因。这项对88名参与者进行的前瞻性队列研究的目的是确定PTB是否与怀孕期间的阴道微生物组相关。在妊娠期间从后穹窿阴道拭子中纯化总DNA。使用聚合酶链反应引物扩增16 S核糖体RNA基因，然后进行链终止测序。通过将重叠群共有序列与核糖体数据库项目进行比较来鉴定细菌。通过比例总结二分响应，通过平均值+/-标准差总结连续变量。平均Shannon多样性指数不同的Welch t检验（P = 0.00016）与PTB和足月妊娠的高加索人。非裔美国人的物种多样性最大（P = .0045）。微生物组/乳酸菌含量的变化和推定的新型/有害细菌的存在与PTB无关。我们得出结论，未培养的阴道细菌在PTB中起着重要作用，种族/种族和采样位置是阴道微生物组的重要决定因素。
Reproductive tract infection is a major initiator of preterm birth (PTB). The objective of this prospective cohort study of 88 participants was to determine whether PTB correlates with the vaginal microbiome during pregnancy. Total DNA was purified from posterior vaginal fornix swabs during gestation. The 16S ribosomal RNA gene was amplified using polymerase chain reaction primers, followed by chain-termination sequencing. Bacteria were identified by comparing contig consensus sequences with the Ribosomal Database Project. Dichotomous responses were summarized via proportions and continuous variables via means +/- standard deviation. Mean Shannon Diversity index differed by Welch t test (P = .00016) between caucasians with PTB and term gestation. Species diversity was greatest among African Americans (P = .0045). Change in microbiome/Lactobacillus content and presence of putative novel/noxious bacteria did not correlate with PTB. We conclude that uncultured vaginal bacteria play an important role in PTB and race/ethnicity and sampling location are important determinants of the vaginal microbiome.