STUDY OF RETENTION PROCESSES IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY BY THE USE OF THE SOLVATOCHROMIC COMPARISON METHOD
STUDY OF RETENTION PROCESSES IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY BY THE USE OF THE SOLVATOCHROMIC COMPARISON METHOD
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DOI：
10.1021/ac00291a049
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发表时间：
1985-01-01
影响因子：
7.4
通讯作者：
ABRAHAM, MH
中科院分区：
文献类型：
--
作者：
SADEK, PC;CARR, PW;ABRAHAM, MH
本文用溶致变色比较法关联了二十几种非亲脂性溶质在反相键合相柱上的保留值。与其他相关研究雅阁，影响保留的最重要的溶质参数是其大小和氢键碱性，而不是氢键酸性。溶质的偶极性是一个次要但重要的因素.保留作为键合相（烃与氟碳化合物），移动的相改性剂（甲醇，乙腈，四氢呋喃），和组合物（70/30甲醇-水至30/70甲醇-水）的性质的函数进行了研究，使用文献和原始数据作为相关性的基础集。所得到的结果表明，任何线性溶剂化能关系的最佳拟合迄今观察到的在许多不同的数据与溶剂化显色parameters.Almost相关自成立以来，一直有许多尝试在键合相反相色谱保留分子的性质。Horvath和他的同事将溶质容量因子与溶质的非极性表面积相关联（1）。Tomlinson和他的同事已经证明辛醇/水分配之间存在线性关系
The retention of some 2 dozen nonsllanophllic solutes on re-versed-phase bonded phase columns has been correlated by the solvatochromic comparison method. In accord with other related studies, the most Important solute parameters, which Influence retention, are Its size and hydrogen bond basicity but not hydrogen bond acidity. The solute’s dipolarity Is a minor but still significant factor. Retention as a function of the nature of the bonded phase (hydrocarbon vs. fluorocarbon), mobile phase modifier (methanol, acetonitrile, tetrahydrofuran), and composition (70/30 methanol-water to 30/70 methanol-water) has been studied using literature and original data as the basis set for the correlations. The results obtained show the best fits of any linear solvation energy relationship thus far observed In correlating many different data with solvatochromic parameters.Almost since its inception, there have been manyattempts to relate retention in bonded phase reversed-phase chromatography to molecular properties. Horvath and his co-workers have related solute capacity factors to the nonpolar surface area of the solute (1). Tomlinson and co-workers have dem-onstrated linear relationships between octanol/water partition