Ran GTPase Regulates Hemocytic Phagocytosis of Shrimp by Interaction with Myosin
Ran GTPase Regulates Hemocytic Phagocytosis of Shrimp by Interaction with Myosin
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Ran GTPase 通过与肌球蛋白相互作用调节虾的血细胞吞噬作用
DOI：
10.1021/pr800840x
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发表时间：
2009-03-01
影响因子：
4.4
通讯作者：
Zhang, Xiaobo
中科院分区：
文献类型：
--
作者：
Liu, Weifeng;Han, Fang;Zhang, Xiaobo
Ran GTPases是小G蛋白超家族中的一个成员，广泛参与细胞质到细胞核的转运系统。然而，Ran GTdR在免疫中的功能尚不清楚。在我们的研究中，我们发现Ran GTdR（命名为PjRan）在抗病毒虾中上调，表明PjRan可能参与了抗病毒感染的先天免疫系统。在蛋白质相互作用的基础上，发现PjRan与吞噬过程中的关键蛋白肌球蛋白相互作用，形成蛋白质复合物。RNA干扰和mRNA检测结果表明，PjRan能够调节对虾血细胞的吞噬功能。进一步的数据表明，PjRan通过RNAi去除导致病毒拷贝数的显著增加，而PjRan的过表达导致病毒拷贝数的显著减少，表明PjRan通过调节吞噬作用参与抗病毒免疫。因此，我们的研究揭示了Ran GT3通过与细胞骨架蛋白直接相互作用而参与吞噬的全新方面，并提出了一种新的抗病毒免疫途径，这将有助于更好地理解无脊椎动物抗病毒感染免疫反应的分子事件。
Ran GTPases, one family of small G protein superfamily, have been widely demonstrated to be involved in the transport system between cytoplasm and the nucleus. However, the function of Ran GTPase in immunity remains unclear. In our study, it was found that the Ran GTPase (designated as PjRan) was up-regulated in virus-resistant shrimp, indicating that the PjRan might be implicated in the innate immune system against virus infection. On the basis of protein interactions, it was found that the PjRan interacted with myosin, a crucial protein in the process of phagocytosis to form a protein complex. The RNAi and mRNA assays showed that the PjRan could regulate shrimp hemocytic phagocytosis. Further data evidenced that the depletion of PjRan by RNAi caused a significant increase of virus copies, and the overexpression of PjRan resulted in a significant decrease of virus copies, suggesting that the PjRan participated in the antiviral immunity by regulating phagocytosis. Therefore, our study revealed a completely novel aspect of Ran GTPase in phagocytosis by the direct interaction with the cytoskeleton protein and presented a novel pathway concerning to antiviral immunity, which will help to better understand the molecular events in immune response against virus infection in invertebrates.