Calcification in Human Intervertebral Disc Degeneration and Scoliosis
Calcification in Human Intervertebral Disc Degeneration and Scoliosis
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DOI：
10.1002/jor.21456
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发表时间：
2011-12-01
影响因子：
2.8
通讯作者：
Mwale, Fackson
中科院分区：
文献类型：
--
作者：
Hristova, Gergana I.;Jarzem, Peter;Mwale, Fackson
钙化是一种病理过程，可导致退行性和脊柱侧凸椎间盘（IVDs）营养供应和椎间盘代谢受损。本研究的目的是评估ivd在退行性椎间盘疾病（DDD）和青少年特发性脊柱侧凸（AIS）中的钙化潜力。为此，从16例成年DDD患者和9例青少年AIS患者的手术中获得34例ivd和25例ivd。对脊柱侧凸椎间盘的凹部和凸部分别进行了分析。Von Kossa染色显示钙沉积，免疫组织化学检测与软骨内成骨相关的X型胶原（COL X）表达。碱性磷酸酶活性、钙和无机磷酸盐浓度作为钙化电位的指标。结果显示，退行性椎间盘和脊柱侧凸椎间盘存在钙沉积和COL X，而对照组椎间盘不存在，且退行性椎间盘和脊柱侧凸椎间盘的钙化电位指标水平始终高于对照组。结果表明，成人椎间盘退变与持续的矿物沉积有关，AIS椎间盘的矿化可能反映了一个过早的退变过程。(C) 2011骨科研究学会。Wiley期刊公司出版。[J]中华口腔外科杂志，2011
Calcification is a pathological process that may lead to impairment of nutrient supply and disc metabolism in degenerative and scoliotic intervertebral discs (IVDs). The purpose of this study was to assess the calcification potential of IVDs in degenerative disc disease (DDD) and adolescent idiopathic scoliosis (AIS). For this purpose, 34 IVDs from 16 adult patients with DDD and 25 IVDs from 9 adolescent patients with AIS were obtained at surgery. The concave and convex parts of the scoliotic discs were analyzed separately. Von Kossa staining was performed to visualize calcium deposits, while type X collagen (COL X) expression associated with endochondral ossification was measured by immunohistochemistry. Alkaline phosphatase activity and calcium and inorganic phosphate concentrations were used as indicators of calcification potential. Results showed the presence of calcium deposits and COL X in degenerative and scoliotic IVDs, but not in control discs, and the level of the indicators of calcification potential was consistently higher in degenerative and scoliotic discs than in control discs. The results suggest that disc degeneration in adults is associated with ongoing mineral deposition and that mineralization in AIS discs might reflect a premature degenerative process. (C) 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29: 1888-1895, 2011