Short-term plasticity at cerebellar granule cell to molecular layer interneuron synapses expands information processing
Short-term plasticity at cerebellar granule cell to molecular layer interneuron synapses expands information processing
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DOI：
10.7554/elife.41586
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发表时间：
2019-05-13
期刊：
影响因子：
7.7
通讯作者：
Doussau, Frederic
中科院分区：
文献类型：
--
作者：
Dorgans, Kevin;Demais, Valerie;Doussau, Frederic
小脑分子层中间神经元（MLI）的信息处理在运动行为中起着至关重要的作用。MLI募集受颗粒细胞（GC）-MLI突触的短时程可塑性（STP）特征的严格控制。虽然GC是大脑中数量最多的神经元，但GC-MLI突触的STP多样性记录很少。在这里，我们研究了在突发放电期间，单个MLI如何被其不同的GC输入招募。使用切片记录在个别GC-MLI突触的小鼠，我们揭示了四类连接隔离的STP配置文件。每个类不同驱动MLI招聘。我们发现GC突触多样性的基础是突触蛋白II的异质性表达，突触蛋白II是STP的关键因素，缺乏突触蛋白II的GC末端与缓慢的MLI募集相关。我们的研究表明，GC末端的分子，结构和功能多样性提供了一种机制，以扩大MLI的编码范围。
Information processing by cerebellar molecular layer interneurons (MLIs) plays a crucial role in motor behavior. MLI recruitment is tightly controlled by the profile of short-term plasticity (STP) at granule cell (GC)-MLI synapses. While GCs are the most numerous neurons in the brain, STP diversity at GC-MLI synapses is poorly documented. Here, we studied how single MLIs are recruited by their distinct GC inputs during burst firing. Using slice recordings at individual GC-MLI synapses of mice, we revealed four classes of connections segregated by their STP profile. Each class differentially drives MLI recruitment. We show that GC synaptic diversity is underlain by heterogeneous expression of synapsin II, a key actor of STP and that GC terminals devoid of synapsin II are associated with slow MLI recruitment. Our study reveals that molecular, structural and functional diversity across GC terminals provides a mechanism to expand the coding range of MLIs.