A simple molecular method for discriminating common filarial nematodes of dogs (Canis familiaris)
A simple molecular method for discriminating common filarial nematodes of dogs (Canis familiaris)
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DOI：
10.1016/j.vetpar.2006.06.006
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发表时间：
2006-11-05
影响因子：
2.6
通讯作者：
Genchi, Claudio
中科院分区：
文献类型：
--
作者：
Casiraghi, Maurizio;Bazzocchi, Chiara;Genchi, Claudio
准确诊断犬丝虫病是选择正确治疗方法的关键。因此，需要可靠的方法来区分狗的不同丝虫病。作者报道了简单和高度特异的分子方法来鉴定三种最常见的欧洲狗丝虫线虫：双丝线虫、爬行丝虫和棘齿线虫(Syn.(1)用普通丝虫引物对线粒体DNA(12S rDNA和Coxi)进行聚合酶链式反应，然后用限制性内切酶进行限制性内切酶消化，从而产生条带多态，明显区分这三个物种；(2)用物种特异性引物进行聚合酶链式反应，以支持限制分析，尤其是在多重感染的情况下。(C)2006爱思唯尔B.V.保留所有权利。
Accurate diagnosis of canine filariosis is essential for choosing correct therapeutic approach. Therefore, reliable methods for discriminating among the different filarial infections in dogs are needed. The authors report simple and highly specific molecular methods that identify the three most common filarial nematodes of European dogs: Dirofilaria immitis, D. repens and Acanthocheilonema (syn. Dipetalonema) reconditum, based on (1) PCR amplifications of mitochondrial DNA (12S rDNA and coxI) with general filarial primers followed by digestion with restriction enzymes that generates band polymorphisms clearly discriminating the three species and (2) PCR amplifications with species-specific primers to support the restriction analysis, in particular in the case of multiple infections. (c) 2006 Elsevier B.V. All rights reserved.