Identification of new potential vaccine candidates against Chlamydia pneumoniae by multiple screenings
Identification of new potential vaccine candidates against Chlamydia pneumoniae by multiple screenings
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DOI：
10.1016/j.vaccine.2004.07.045
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发表时间：
2005-01-19
期刊：
影响因子：
5.5
通讯作者：
Grandi, G
中科院分区：
文献类型：
--
作者：
Finco, O;Bonci, A;Grandi, G
衣原体是与严重人类疾病相关的细胞内细菌。迄今为止，事实证明疫苗很难获得，目前的观点认为可能需要多抗原组合才能诱导最佳保护反应。为了识别新的潜在候选疫苗，我们最近筛选了肺炎衣原体 (Cpn) 基因组，并描述了 53 种重组蛋白，这些蛋白可引发抗体与纯化的 Cpn 细胞结合。我们现在报道该组中的六种蛋白质也可以诱导体外中和抗体。通过 2DE Cpn 蛋白图谱的免疫印迹分析评估相应抗原的抗体特异性。此外，六种体外中和抗原中的四种（Pmp2、Pmp 10、OmpH 样和烯醇化酶）可以抑制仓鼠模型中的 Cpn 传播。结果表明，这些 Cpn 蛋白在传染性 EB 中是免疫可及的，并建议进一步研究它们作为疫苗成分的价值。 (C) 2004 Elsevier Ltd. 保留所有权利。
Chlamydia are intracellular bacteria associated to serious human disease. A vaccine has proved difficult to obtain so far, and current opinions agree that multi-antigen combinations may be required to induce optimal protective responses. In order to identify new potential vaccine candidates, we recently screened the Chlamydia pneumoniae (Cpn) genome and described 53 recombinant proteins which elicited antibodies binding to purified Cpn cells. We now report that six proteins in this group can also induce in vitro neutralizing antibodies. Antibody specificity for the corresponding antigens was assessed by immunoblot analysis of 2DE Cpn protein maps. Furthermore, four of the six in vitro neutralizing antigens (Pmp2, Pmp 10, OmpH-like and enolase) could inhibit Cpn dissemination in a hamster model. The results show that these Cpn proteins are immunoaccessible in infectious EBs, and recommend further investigation on their value as vaccine components. (C) 2004 Elsevier Ltd. All rights reserved.