P2Z adenosine triphosphate receptor activity in cultured human monocyte-derived macrophages.
P2Z adenosine triphosphate receptor activity in cultured human monocyte-derived macrophages.
复制标题
DOI：
10.1182/blood.v84.8.2452.bloodjournal8482452
复制
发表时间：
1994-10
期刊：
影响因子：
20.3
通讯作者：
Suzanne E. Hickman;J. E. Khoury;Steven Greenberg;I. Schieren;Samuel C. Silverstein
中科院分区：
文献类型：
--
作者：
Suzanne E. Hickman;J. E. Khoury;Steven Greenberg;I. Schieren;Samuel C. Silverstein
本研究表明，人单核吞噬细胞表达p2z样嘌呤能膜受体活性。细胞外三磷酸腺苷（ATP）在人单核吞噬细胞中诱导非选择性膜孔的形成，使膜上不需要的荧光染料（YO-PRO-1或路西法黄）进入这些细胞的细胞质。单核吞噬细胞被ATP渗透的百分比随着单核细胞成熟为巨噬细胞而增加。Mg2+和氧化ATP抑制了它们对ATP的反应。Benzoylbenzoic-ATP （BzBzATP）在使人巨噬细胞渗透到YO-PRO-1或路西法黄上的有效性约为ATP的60%，腺苷-5 - o -（硫代磷酸）（ATP γ S）的有效性低于ATP的20%。因此，人类p2z样受体与小鼠受体不同，因为BzBzATP、ATP和ATP γ S在使小鼠巨噬细胞样J774细胞渗透这些染料方面同样有效。UTP、GTP和CTP在人或小鼠巨噬细胞对YO-PRO-1的渗透作用中无效。综上所述，这些数据表明，人类单核巨噬细胞表达的p2z样活性在药理学上不同于小鼠同类细胞表达的p2z样活性，并且这些受体的表达在人类单核吞噬细胞中受到发育调节。
The present study shows that human mononuclear phagocytes express a P2Z-like purinergic membrane receptor activity. Extracellular adenosine triphosphate (ATP) induces the formation of nonselective membrane pores in human mononuclear phagocytes that allow the entry of otherwise membrane impermeant fluorescent dyes (YO-PRO-1 or Lucifer yellow) into the cytoplasm of these cells. The percentage of mononuclear phagocytes that was permeabilized by ATP increased as monocytes matured into macrophages. Their response to ATP was inhibited by Mg2+ and oxidized ATP. Benzoylbenzoic-ATP (BzBzATP) was approximately 60% as effective as ATP and adenosine-5 -O-(thiophosphate) (ATP gamma S) was less than 20% as effective as ATP in permeabilizing human macrophages to YO-PRO-1 or Lucifer Yellow. Thus, the human P2Z-like receptor differs from its murine counterpart because BzBzATP, ATP, and ATP gamma S are equally efficacious in permeabilizing murine macrophage-like J774 cells to these dyes. UTP, GTP, and CTP were ineffective in permeabilizing human or murine macrophages to YO-PRO-1. Taken together, these data indicate that human monocyte-derived macrophages express a P2Z-like activity that is pharmacologically distinct from that expressed by their murine counterparts and that expression of these receptors is developmentally regulated in human mononuclear phagocytes.