LOCALIZATION OF MITOCHONDRIA IN LIVING CELLS WITH RHODAMINE-123
LOCALIZATION OF MITOCHONDRIA IN LIVING CELLS WITH RHODAMINE-123
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DOI：
10.1073/pnas.77.2.990
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
CHEN, LB
中科院分区：
文献类型：
--
作者：
JOHNSON, LV;WALSH, ML;CHEN, LB
激光染料罗丹明123是活细胞中线粒体定位的特异性探针。凭借其对线粒体的选择性及其荧光特性，用罗丹明123染色的线粒体的可检测性比通过常规光显微镜技术提供的可检测性显著提高。随着罗丹明123的使用，它是可能的，以检测线粒体分布的改变劳氏肉瘤病毒和秋水仙碱处理诱导的线粒体的形状和组织的变化转化后。
The laser dye rhodamine 123 is a specific probe for the localization of mitochondria in living cells. By virtue of its selectivity for mitochondria and its fluorescent properties, the detectability of mitochondria stained with rhodamine 123 is significantly improved over that provided by conventional light microscopic techniques. With the use of rhodamine 123, it is possible to detect alterations in mitochondrial distribution following transformation by Rous sarcoma virus and changes in the shape and organization of mitochondria induced by colchicine treatment.