Evolutionary relationships among Japanese pond frogs inferred from mitochondrial DNA sequences of cytochrome b and 12S ribosomal RNA genes.
Evolutionary relationships among Japanese pond frogs inferred from mitochondrial DNA sequences of cytochrome b and 12S ribosomal RNA genes.
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从细胞色素 b 和 12S 核糖体 RNA 基因的线粒体 DNA 序列推断日本池塘青蛙的进化关系。
DOI：
10.1266/ggs.73.121
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发表时间：
1998
影响因子：
1.1
通讯作者：
H. Yonekawa
中科院分区：
文献类型：
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作者：
M. Sumida;M. Ogata;H. Kaneda;H. Yonekawa
日本池蛙（黑斑蛙、多孔蛙和多孔蛙）之间的进化关系。通过分析线粒体细胞色素B（cyt B）和12 S rRNA基因的核苷酸序列，研究了短足拟步行虫（P. brevipoda）的线粒体细胞色素B（cyt B）和12 S rRNA基因的核苷酸序列。采用PCR-直接测序法测定了日本池蛙13个种群18只的cyt B基因444 bp片段和12 S rRNA基因410 bp片段的核苷酸序列，并采用邻接法和最大似然法构建了系统发育树。catesbeiana作为一个外群体。cyt B基因444-bp片段的测序结果提供了69个变异位点，12 S rRNA基因410-bp片段的测序结果提供了21个变异位点。cyt B基因在群体水平、种或亚种水平和种属水平上的核苷酸替换数分别为0.0022-0.0205、0.0368-0.0462和0.1038-0.1244; 12 S rRNA基因在群体或亚种水平上的核苷酸替换数分别为0-0.0074。0.0378-0.0456。内群内的核苷酸替换主要发生在cyt B基因的第3个密码子位置，为沉默突变。内群内cyt B和12 S rRNA基因的转换频率高于颠换。根据cyt B基因核苷酸序列构建的系统发育树表明，在外群R.内群日本池蛙分化为黑斑蛙和孔蛙，孔蛙又分化为孔蛙、短足蛙（典型的冈山种）和孔蛙的名古屋种。根据12 S rRNA基因核苷酸序列构建的系统发育树也显示了两个物种之间的明显分歧，但没有任何物种内的分歧。
The evolutionary relationships among Japanese pond frogs (Rana nigromaculata, R.porosa porosa, and R. p. brevipoda) were investigated by analyzing nucleotide sequences of mitochondrial cytochrome b (cyt b) and 12S rRNA genes. The nucleotide sequences of 444-bp segment of the cyt b gene and 410-bp segment of 12S rRNA gene were determined by the PCR-direct sequencing method using 18 frogs from 13 populations of Japanese pond frogs, and phylogenetic trees were constructed by the neighbor-joining and maximum likelihood methods using R. catesbeiana as an outgroup. The sequenced 444-bp segment of cyt b gene provided 69 variables sites, and the sequenced 410-bp segment of 12S rRNA gene provided 21 variables sites. The numbers of nucleotide substitutions per site of the cyt b gene within ingroup were 0.0022-0.0205 at the populational level, 0.0368-0.0462 at the racial or subspecific level, and 0.1038-0.1244 at the specific level, whereas those of the 12S rRNA gene were 0-0.0074 at the populational or subspecific level, and 0.0378-0.0456 at the specific level. Most nucleotide substitutions within ingroup occurred at the third codon position of the cyt b gene and were silent mutations. High frequencies of transitions relative to transversions were shown at cyt b and 12S rRNA genes within ingroup. The phylogenetic trees constructed from the nucleotide sequences of the cyt b gene showed that after outgroup R. catesbeiana separated from ingroup frogs, ingroup Japanese pond frogs diverged into R.nigromaculata and R.porosa, then the latter diverged into R.p. porosa, R.p. brevipoda (the typical Okayama race), and the Nagoya race of R.p.porosa. The phylogenetic trees constructed from the nucleotide sequences of the 12S rRNA gene also showed distinct divergence between two species, but not any divergence within species.
Iida, R.，：“使用金-硫化物-银强化过氧化物酶-二氨基联苯胺染色：一种快速且高度灵敏的免疫印迹方法”电泳。
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