A Large-Scale Semi-Chronic Microdrive Recording System for Non-Human Primates
A Large-Scale Semi-Chronic Microdrive Recording System for Non-Human Primates
复制标题
DOI：
10.1016/j.neuron.2017.09.050
复制
发表时间：
2017-11-15
期刊：
影响因子：
16.2
通讯作者：
Gray, Charles M.
中科院分区：
文献类型：
--
作者：
Dotson, Nicholas M.;Hoffman, Steven J.;Gray, Charles M.
非人类灵长类动物的多电极记录为测量大脑功能基础的广泛分布的活动模式提供了一种关键方法。然而，常见的技术依赖于小的、通常不可移动的阵列或微驱动器，其仅能够操纵少量的紧密间隔的探针。这些技术限制了可以同时采样的皮层区域的数量，并且通常不能到达皮层下目标。为了克服这些局限性，我们开发了一个大规模的，半慢性微驱动记录系统与多达256个独立可移动的微电极跨越整个大脑半球。微驱动器系统是密封的，没有内部连接线，并已被用来同时记录多达37个皮层和皮层下的清醒行为的猴子长达9个月。作为一个原则的证明，我们证明了这种技术的能力，以解决网络级的问题，使用功能连接数据的图论分析。
Multi-electrode recordings in the non-human primate provide a critical method for measuring the widely distributed activity patterns that underlie brain function. However, common techniques rely on small, often immovable arrays, or microdrives, that are only capable of manipulating a small number of closely spaced probes. These techniques restrict the number of cortical areas that can be simultaneously sampled and are typically not capable of reaching subcortical targets. To overcome these limitations, we developed a large-scale, semi-chronic microdrive recording system with up to 256 independently movable microelectrodes spanning an entire cerebral hemisphere. The microdrive system is hermetically sealed, free of internal connecting wires, and has been used to simultaneously record from up to 37 cortical and subcortical areas in awake behaving monkeys for up to 9 months. As a proof of principle, we demonstrate the capability of this technique to address network-level questions using a graph theoretic analysis of functional connectivity data.