Isolation of a full-length human WNT7A gene implicated in limb development and cell transformation, and mapping to chromosome 3p25
Isolation of a full-length human WNT7A gene implicated in limb development and cell transformation, and mapping to chromosome 3p25
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DOI：
10.1016/s0378-1119(96)00808-6
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发表时间：
1997-04-11
期刊：
影响因子：
3.5
通讯作者：
Harris, AL
中科院分区：
文献类型：
--
作者：
Bui, TD;Lako, M;Harris, AL
Wnt基因家族在发育和肿瘤发生中起作用。一个小鼠成员Wnt 7a对体内肢体发育至关重要，并且在体外也具有转化能力。本研究报道了通过文库筛选分离小鼠Wnt 7a基因的全长人类同源物。酵母人工染色体荧光原位杂交（YAC-FISH）将WNT 7A基因定位于染色体3 p25。人WNT 7A的ORF编码349个氨基酸，与小鼠WNT 7a在氨基酸和核酸水平上的同源性分别为97%和92%。它具有22个保守的半胱氨酸残基和3个以上的氨基末端，和一个假定的聚腺苷酸尾。这是第五个已确定完整cDNA序列的人WNT基因。(C)1997年Elsevier Science B.V.
The Wnt gene family has a role in development as well as tumourigenesis. One mouse member, Wnt7a, is vital for limb development in vivo and also possesses transforming ability in vitro. This study reports the isolation of a full length of human homologue of mouse Wnt7a gene by library screening. Yeast artificial chromosome-fluorescence in situ hybridisation (YAC-FISH) mapped the WNT7A gene to chromosome 3p25. Human WNT7A had an ORF encoding a deduced protein of 349 aa that exhibited 97% and 92% identity to mouse Wnt7a at the aa and nucleic acid levels, respectively. It possessed the 22 conserved cysteine residues and 3 more at the amino terminus, and a putative poly A tail. This is the fifth human WNT gene in which a complete cDNA sequence had been determined. (C) 1997 Elsevier Science B.V.